Literature DB >> 16725156

Interplay between the beta' clamp and the beta' jaw domains during DNA opening by the bacterial RNA polymerase at sigma54-dependent promoters.

Siva R Wigneshweraraj1, Dhruti Savalia, Konstantin Severinov, Martin Buck.   

Abstract

The bacterial RNA polymerase (RNAP) is a multi-subunit, structurally flexible, complex molecular machine, in which activities associated with DNA opening for transcription-competent open promoter complex (OC) formation reside in the catalytic beta and beta' subunits and the dissociable sigma subunit. OC formation is a multi-step process that involves several structurally conserved mobile modules of beta, beta', and sigma. Here, we present evidence that two flexible modules of beta', the beta' jaw and the beta' clamp and a conserved regulatory Region I domain of sigma(54), jointly contribute to the maintenance of stable DNA strand separation around the trancription start site in OCs formed at sigma(54)-dependent promoters. Clearly, regulated interplay between the mobile modules of the beta' and the sigma subunits of the RNAP appears to be necessary for stable OC formation.

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Substances：

Year:  2006        PMID： 16725156     DOI： 10.1016/j.jmb.2006.04.063

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  6 in total

1.  Real-time footprinting of DNA in the first kinetically significant intermediate in open complex formation by Escherichia coli RNA polymerase.

Authors:  Caroline A Davis; Craig A Bingman; Robert Landick; M Thomas Record; Ruth M Saecker
Journal:  Proc Natl Acad Sci U S A       Date:  2007-04-30       Impact factor: 11.205

2.  Structural and mechanistic basis for the inhibition of Escherichia coli RNA polymerase by T7 Gp2.

Authors:  Ellen James; Minhao Liu; Carol Sheppard; Vladimir Mekler; Beatriz Cámara; Bing Liu; Pete Simpson; Ernesto Cota; Konstantin Severinov; Steve Matthews; Sivaramesh Wigneshweraraj
Journal:  Mol Cell       Date:  2012-07-19       Impact factor: 17.970

3.  Construction and functional analyses of a comprehensive sigma54 site-directed mutant library using alanine-cysteine mutagenesis.

Authors:  Yan Xiao; Siva R Wigneshweraraj; Robert Weinzierl; Yi-Ping Wang; Martin Buck
Journal:  Nucleic Acids Res       Date:  2009-05-27       Impact factor: 16.971

4.  Coupling sigma factor conformation to RNA polymerase reorganisation for DNA melting.

Authors:  Patricia C Burrows; Nicolas Joly; Wendy V Cannon; Beatriz P Cámara; Mathieu Rappas; Xiaodong Zhang; Kathleen Dawes; B Tracy Nixon; Siva R Wigneshweraraj; Martin Buck
Journal:  J Mol Biol       Date:  2009-01-31       Impact factor: 5.469

5.  A dual switch controls bacterial enhancer-dependent transcription.

Authors:  Simone C Wiesler; Patricia C Burrows; Martin Buck
Journal:  Nucleic Acids Res       Date:  2012-09-10       Impact factor: 16.971

6.  Structures of Bacterial RNA Polymerase Complexes Reveal the Mechanism of DNA Loading and Transcription Initiation.

Authors:  Robert Glyde; Fuzhou Ye; Milija Jovanovic; Ioly Kotta-Loizou; Martin Buck; Xiaodong Zhang
Journal:  Mol Cell       Date:  2018-06-21       Impact factor: 17.970
  6 in total

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