OBJECTIVE: To evaluate four ELISAs (commercialized by Panbio and Serion) for IgM and IgG detection in an outbreak of Q fever. METHODS: Fifty-three serum samples corresponding to 29 persons were processed. An indirect immunofluorescence assay was used as the reference method. RESULTS: The sensitivity and specificity for IgM were 100% and 74% (Panbio), and 91% and 88% (Serion). IgG sensitivity was 72% (Panbio) and 89% (Serion). The specificity of both techniques for IgG was 97%. CONCLUSIONS: Both ELISAs seem to be good IgM screening methods for use during Q fever outbreaks.
OBJECTIVE: To evaluate four ELISAs (commercialized by Panbio and Serion) for IgM and IgG detection in an outbreak of Q fever. METHODS: Fifty-three serum samples corresponding to 29 persons were processed. An indirect immunofluorescence assay was used as the reference method. RESULTS: The sensitivity and specificity for IgM were 100% and 74% (Panbio), and 91% and 88% (Serion). IgG sensitivity was 72% (Panbio) and 89% (Serion). The specificity of both techniques for IgG was 97%. CONCLUSIONS: Both ELISAs seem to be good IgM screening methods for use during Q fever outbreaks.
Authors: Joseph Royal; Mark S Riddle; Emad Mohareb; Marshall R Monteville; Chad K Porter; Dennis J Faix Journal: Am J Trop Med Hyg Date: 2013-09-16 Impact factor: 2.345