| Literature DB >> 16719383 |
Thomas K H Chang1, Charles L Crespi, David J Waxman.
Abstract
The cytochrome P450 enzyme CYP2E1 catalyzes the oxidative metabolism of many solvents and other small organic molecules. A spectrophotometric method is described for determination of CYP2E1 activity by monitoring the formation of p-nitrocatechol from p-nitrophenol by cDNA-expressed CYP2E1 or isolated liver microsomes. The enzymatic product, p-nitrocatechol, is assayed at 535 nm after acidification of the reaction mixture with trichloroacetic acid followed by neutralization using 2 M NaOH. This method is applicable to enzymatic studies for determination of P450-catalyzed p-nitrophenol hydroxylation activity.Entities:
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Year: 2006 PMID： 16719383 DOI： 10.1385/1-59259-998-2:127
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745