Literature DB >> 16719371

Establishment of functional human cytochrome P450 monooxygenase systems in Escherichia coli.

Michael P Pritchard1, Lesley McLaughlin, Thomas Friedberg.   

Abstract

Cytochromes P450 (CYP) have been expressed in a variety of systems such as mammalian cells, yeast, and bacteria. The bacterial system is technically the least demanding and provides large amounts of catalytically active P450s for metabolic and structural studies relating to preclinical drug development. This chapter provides a detailed technical description of the processes that allow the coexpression of various CYP isoforms together with CYP reductase in Escherichia coli and gives some examples of the results that can be achieved for the expression of human P450s.

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Year:  2006        PMID： 16719371     DOI： 10.1385/1-59259-998-2:19

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  16 in total

1.  Heterologous expression of human cytochromes P450 2D6 and CYP3A4 in Escherichia coli and their functional characterization.

Authors:  Yan Pan; Badrul Amini Abd-Rashid; Zakiah Ismail; Rusli Ismail; Joon Wah Mak; Chin Eng Ong
Journal:  Protein J       Date:  2011-12       Impact factor: 2.371

2.  A novel CYP2A6 allele, CYP2A6*23, impairs enzyme function in vitro and in vivo and decreases smoking in a population of Black-African descent.

Authors:  Man Ki Ho; Jill C Mwenifumbo; Bin Zhao; Elizabeth M J Gillam; Rachel F Tyndale
Journal:  Pharmacogenet Genomics       Date:  2008-01       Impact factor: 2.089

3.  Cytochrome P450-mediated 17beta-estradiol metabolism in zebrafish (Danio rerio).

Authors:  Marcus L Scornaienchi; Cammi Thornton; Kristine L Willett; Joanna Y Wilson
Journal:  J Endocrinol       Date:  2010-06-03       Impact factor: 4.286

4.  Functional differences in the cytochrome P450 1 family enzymes from zebrafish (Danio rerio) using heterologously expressed proteins.

Authors:  Marcus L Scornaienchi; Cammi Thornton; Kristine L Willett; Joanna Y Wilson
Journal:  Arch Biochem Biophys       Date:  2010-06-18       Impact factor: 4.013

5.  Identification and validation of a gene causing cross-resistance between insecticide classes in Anopheles gambiae from Ghana.

Authors:  Sara N Mitchell; Bradley J Stevenson; Pie Müller; Craig S Wilding; Alexander Egyir-Yawson; Stuart G Field; Janet Hemingway; Mark J I Paine; Hilary Ranson; Martin James Donnelly
Journal:  Proc Natl Acad Sci U S A       Date:  2012-03-28       Impact factor: 11.205

6.  Chemical toxicity testing in vitro using cytochrome P450-expressing cell lines, such as human CYP1B1.

Authors:  Robert Landsiedel; Eric Fabian; Tewes Tralau; Andreas Luch
Journal:  Nat Protoc       Date:  2011-04-28       Impact factor: 13.491

7.  Allelic Variation of Cytochrome P450s Drives Resistance to Bednet Insecticides in a Major Malaria Vector.

Authors:  Sulaiman S Ibrahim; Jacob M Riveron; Jaclyn Bibby; Helen Irving; Cristina Yunta; Mark J I Paine; Charles S Wondji
Journal:  PLoS Genet       Date:  2015-10-30       Impact factor: 5.917

8.  Cunninghamella as a microbiological model for metabolism of histamine H(3) receptor antagonist 1-[3-(4-tert-butylphenoxy)propyl]piperidine.

Authors:  Elżbieta Pękala; Paulina Kubowicz; Dorota Łażewska
Journal:  Appl Biochem Biotechnol       Date:  2012-09-16       Impact factor: 2.926

9.  Field-caught permethrin-resistant Anopheles gambiae overexpress CYP6P3, a P450 that metabolises pyrethroids.

Authors:  Pie Müller; Emma Warr; Bradley J Stevenson; Patricia M Pignatelli; John C Morgan; Andrew Steven; Alexander E Yawson; Sara N Mitchell; Hilary Ranson; Janet Hemingway; Mark J I Paine; Martin J Donnelly
Journal:  PLoS Genet       Date:  2008-11-28       Impact factor: 5.917

10.  The cytochrome P450 CYP6P4 is responsible for the high pyrethroid resistance in knockdown resistance-free Anopheles arabiensis.

Authors:  Sulaiman S Ibrahim; Jacob M Riveron; Robert Stott; Helen Irving; Charles S Wondji
Journal:  Insect Biochem Mol Biol       Date:  2015-11-06       Impact factor: 4.714
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