Literature DB >> 1671853

Detection of complex alleles by direct analysis of DNA heteroduplexes.

R Sorrentino1, C Iannicola, S Costanzi, A Chersi, R Tosi.   

Abstract

DNA molecules derived from three alleles of the HLA-DRB3 locus and differing from each other at several nucleotide sites were denatured and cross-hybridized. Each allelic combination was found to generate a pair of heteroduplexes of different mobility. Their retardation as compared to homoduplexes was proportional to the number of mismatches. In each heteroduplexes pair the component possessing the highest number of Pyr-Pyr oppositions was the most retarded. The results are those predicted by a theoretical model implying a correlation between base-pair opening and bending of the DNA double helix. These observations introduce a new HLA typing method at the genomic level and indicate an experimental approach to the analysis of the superhelical DNA conformation as related to different types of base oppositions.

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Year:  1991        PMID： 1671853     DOI： 10.1007/bf00210825

Source DB:  PubMed          Journal:  Immunogenetics        ISSN： 0093-7711            Impact factor:   2.846


  21 in total

1.  Complementary base pairing and the origin of substitution mutations.

Authors:  M D Topal; J R Fresco
Journal:  Nature       Date:  1976-09-23       Impact factor: 49.962

2.  HLA-DRB nucleotide sequences, 1990.

Authors:  S G Marsh; J G Bodmer
Journal:  Immunogenetics       Date:  1990       Impact factor: 2.846

3.  DNA typing of an HLA-DR bilocus specificity by gene amplification and oligonucleotide hybridization.

Authors:  R Sorrentino; C Iannicola; S Botti; S Costanzi; N Tanigaki; R Tosi
Journal:  Immunogenetics       Date:  1989       Impact factor: 2.846

4.  The intrinsic curvature of DNA in solution.

Authors:  C R Calladine; H R Drew; M J McCall
Journal:  J Mol Biol       Date:  1988-05-05       Impact factor: 5.469

5.  Allelic variation in the DR subregion of the human major histocompatibility complex.

Authors:  J I Bell; D Denney; L Foster; T Belt; J A Todd; H O McDevitt
Journal:  Proc Natl Acad Sci U S A       Date:  1987-09       Impact factor: 11.205

6.  Detection of single base substitutions in total genomic DNA.

Authors:  R M Myers; N Lumelsky; L S Lerman; T Maniatis
Journal:  Nature       Date:  1985 Feb 7-13       Impact factor: 49.962

7.  Pyrimidine.pyrimidine base-pair mismatches in DNA. A nuclear magnetic resonance study of T.T pairing at neutral pH and C.C pairing at acidic pH in dodecanucleotide duplexes.

Authors:  M Kouchakdjian; B F Li; P F Swann; D J Patel
Journal:  J Mol Biol       Date:  1988-07-05       Impact factor: 5.469

8.  HLA-DP typing by DNA amplification and hybridization with specific oligonucleotides.

Authors:  G Angelini; T L Bugawan; L Delfino; H A Erlich; G B Ferrara
Journal:  Hum Immunol       Date:  1989-11       Impact factor: 2.850

9.  Structure of an adenine-cytosine base pair in DNA and its implications for mismatch repair.

Authors:  W N Hunter; T Brown; N N Anand; O Kennard
Journal:  Nature       Date:  1986 Apr 10-16       Impact factor: 49.962

10.  Complete sequence of an HLA-dR beta chain deduced from a cDNA clone and identification of multiple non-allelic DR beta chain genes.

Authors:  E O Long; C T Wake; J Gorski; B Mach
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
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  6 in total

1.  Frequency of donor cytotoxic T cell precursors does not correlate with occurrence of acute graft-versus-host disease in children transplanted using unrelated donors.

Authors:  D Montagna; R Maccario; P Comoli; L Prete; M Zecca; E Giraldi; C Daielli; A Moretta; P De Stefano; F Locatelli
Journal:  J Clin Immunol       Date:  1996-03       Impact factor: 8.317

2.  Single base mutations can be unequivocally and rapidly detected by analysis of DNA heteroduplexes, obtained with deletion-mutant instead of wild-type DNA.

Authors:  E van den Akker; J E Braun; G Pals; M V Lafleur; J Retèl
Journal:  Nucleic Acids Res       Date:  1992-12-25       Impact factor: 16.971

3.  Elimination of heteroduplex artifacts when sequencing HLA genes amplified by polymerase chain reaction (PCR).

Authors:  D L'Abbé; A Belmaaza; F Décary; P Chartrand
Journal:  Immunogenetics       Date:  1992       Impact factor: 2.846

4.  Short insertions in the partner strands greatly enhance the discriminating power of DNA heteroduplex analysis: resolution of HLA-DQB1 polymorphisms.

Authors:  M D'Amato; R Sorrentino
Journal:  Nucleic Acids Res       Date:  1995-06-11       Impact factor: 16.971

5.  A new approach to HLA-DPB1 typing combining DNA heteroduplex analysis with allele-specific amplification and enzyme restriction.

Authors:  R Sorrentino; I Potolicchio; G B Ferrara; R Tosi
Journal:  Immunogenetics       Date:  1992       Impact factor: 2.846

6.  Initial characterization of a protochordate histocompatibility locus.

Authors:  Anthony W De Tomaso; Irving L Weissman
Journal:  Immunogenetics       Date:  2003-10-01       Impact factor: 2.846
  6 in total

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