Literature DB >> 1671715

Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: intracellular targeting to the amyloplast.

M B Singh1, T Hough, P Theerakulpisut, A Avjioglu, S Davies, P M Smith, P Taylor, R J Simpson, L D Ward, J McCluskey.   

Abstract

We have identified a major allergenic protein from rye-grass pollen, tentatively designated Lol pIb of 31kDa and with pI 9.0. A cDNA clone encoding Lol pIb has been isolated, sequenced, and characterized. Lol pIb is located mainly in the starch granules. This is a distinct allergen from Lol pI, which is located in the cytosol. Lol pIb is synthesized in pollen as a pre-allergen with a transit peptide targeting the allergen to amyloplasts. Epitope mapping of the fusion protein localized the IgE binding determinant in the C-terminal domain.

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Year:  1991        PMID： 1671715      PMCID： PMC51022          DOI： 10.1073/pnas.88.4.1384

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  21 in total

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Authors:  G P Cottam; D M Moran; R Standring
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7.  A simple and very efficient method for generating cDNA libraries.

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Journal:  Int Arch Allergy Appl Immunol       Date:  1985

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10.  Cross-reactive and unique grass group I antigenic determinants defined by monoclonal antibodies.

Authors:  R E Esch; D G Klapper
Journal:  J Allergy Clin Immunol       Date:  1987-03       Impact factor: 10.793
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  19 in total

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Review 4.  Modulation of allergen-specific antibody responses by T-cell-based peptide vaccine(s). Principles and potential.

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9.  Arabidopsis type B monogalactosyldiacylglycerol synthase genes are expressed during pollen tube growth and induced by phosphate starvation.

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