| Literature DB >> 16716302 |
Mohammad Ali Oshaghi1, Ali Reza Chavshin, Hassan Vatandoost.
Abstract
An important variable in the amplification of arthropod vector-borne diseases is the degree of contact between human hosts and mosquito vectors. To analyze this interaction, a DNA based method was developed to differentiate human bloodmeals from other sources in the mosquito Anopheles stephensi (Diptera: Culicidae) Liston. A portion of the host mitochondrial DNA cytochrome B genes were PCR amplified and classified to the species level based on their restriction fragment length polymorphism (RFLP). The cytochrome B sequences showed sufficient interspecific polymorphism to distinguish between human, cow, sheep, chicken, and guinea pig hosts. XhoI could distinguish human from other vertebrates whereas TaqI alone could separate the others. The importance of these results in epidemiological studies of malaria and other vector borne diseases is discussed.Entities:
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Year: 2006 PMID： 16716302 DOI： 10.1016/j.exppara.2006.04.001
Source DB: PubMed Journal: Exp Parasitol ISSN： 0014-4894 Impact factor: 2.011