| Literature DB >> 16713002 |
Giorgos Sianidis1, Sven-Eric Wohlert, Charalambos Pozidis, Spyridoula Karamanou, Andriy Luzhetskyy, Andreas Vente, Anastassios Economou.
Abstract
We have cloned the gene that encodes a novel glucosyl transferase (AraGT) involved in rhamnosylation of the polyketide antibiotic Aranciamycin in Streptomyces echinatus. AraGT comprises two domains characteristic of bacterial glycosyltranferases. AraGT was synthesized in E. coli as a decahistidinyl-tagged polypeptide. Purified AraGT is dimeric, displays a T(mapp) of 30 degrees C and can glycosylate the aglycone of an Aranciamycin derivative as shown by liquid chromatography and mass spectrometry. The availability of functional AraGT will allow the generation Aranciamycin-based combinatorial libraries.Entities:
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Year: 2006 PMID： 16713002 DOI： 10.1016/j.jbiotec.2006.03.035
Source DB: PubMed Journal: J Biotechnol ISSN： 0168-1656 Impact factor: 3.307