Literature DB >> 1671203

A rapid method for the isolation of genomic DNA from citrated whole blood.

S Parzer1, C Mannhalter.   

Abstract

We have developed a DNA isolation method which allows the isolation of high quality DNA from frozen citrated blood and cell lines within 90 min. The procedure avoids the use of toxic organic reagents and tedious extraction steps. DNA prepared by this method is very well suited for restriction fragment length polymorphism and polymerase chain reaction analysis.

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Year:  1991        PMID： 1671203      PMCID： PMC1149904          DOI： 10.1042/bj2730229

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  9 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Purification of human genomic DNA from whole blood using sodium perchlorate in place of phenol.

Authors:  M B Johns; J E Paulus-Thomas
Journal:  Anal Biochem       Date:  1989-08-01       Impact factor: 3.365

3.  Analysis of human Y-chromosome-specific reiterated DNA in chromosome variants.

Authors:  L M Kunkel; K D Smith; S H Boyer; D S Borgaonkar; S S Wachtel; O J Miller; W R Breg; H W Jones; J M Rary
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205

4.  Isolation of genomic DNA.

Authors:  B G Herrmann; A M Frischauf
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

5.  An improved method for prenatal diagnosis of genetic diseases by analysis of amplified DNA sequences. Application to hemophilia A.

Authors:  S C Kogan; M Doherty; J Gitschier
Journal:  N Engl J Med       Date:  1987-10-15       Impact factor: 91.245

6.  A rapid method for the purification of DNA from blood.

Authors:  M Jeanpierre
Journal:  Nucleic Acids Res       Date:  1987-11-25       Impact factor: 16.971

7.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

8.  Rapid isolation of eukaryotic DNA.

Authors:  D D Bowtell
Journal:  Anal Biochem       Date:  1987-05-01       Impact factor: 3.365

9.  Construction of cDNA coding for human von Willebrand factor using antibody probes for colony-screening and mapping of the chromosomal gene.

Authors:  C L Verweij; C J de Vries; B Distel; A J van Zonneveld; A G van Kessel; J A van Mourik; H Pannekoek
Journal:  Nucleic Acids Res       Date:  1985-07-11       Impact factor: 16.971
  9 in total
  9 in total

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Authors:  Chew-Kiat Heng; Suman Lal; Nilmani Saha; Poh-Sim Low; M Ilyas Kamboh
Journal:  Hum Genet       Date:  2003-10-31       Impact factor: 4.132

2.  Compound heterozygosity of novel missense mutations in the gamma-glutamyl-carboxylase gene causes hereditary combined vitamin K-dependent coagulation factor deficiency.

Authors:  Dhouha Darghouth; Kevin W Hallgren; Rebecca L Shtofman; Amel Mrad; Youssef Gharbi; Ahmed Maherzi; Radhia Kastally; Sophie LeRicousse; Kathleen L Berkner; Jean-Philippe Rosa
Journal:  Blood       Date:  2006-05-23       Impact factor: 22.113

3.  A rapid and efficient DNA extraction protocol from fresh and frozen human blood samples.

Authors:  Pokhraj Guha; Avishek Das; Somit Dutta; Tapas Kumar Chaudhuri
Journal:  J Clin Lab Anal       Date:  2017-02-23       Impact factor: 2.352

4.  ABCA1 gene polymorphisms and their associations with coronary artery disease and plasma lipids in males from three ethnic populations in Singapore.

Authors:  Jenny Hui-Hui Tan; Poh-Sim Low; Yong-Seng Tan; Ming-Chuan Tong; Nilmani Saha; Hongyuan Yang; Chew-Kiat Heng
Journal:  Hum Genet       Date:  2003-04-23       Impact factor: 4.132

5.  Acyl-CoA: cholesterol acyltransferase-2 gene polymorphisms and their association with plasma lipids and coronary artery disease risks.

Authors:  Xuelian He; Yongjian Lu; Nilmani Saha; Hongyuan Yang; Chew-Kiat Heng
Journal:  Hum Genet       Date:  2005-09-30       Impact factor: 4.132

6.  DNA polymorphisms in two paraoxonase genes (PON1 and PON2) are associated with the risk of coronary heart disease.

Authors:  D K Sanghera; C E Aston; N Saha; M I Kamboh
Journal:  Am J Hum Genet       Date:  1998-01       Impact factor: 11.025

7.  Polymorphism (C677T) in the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene: A preliminary study on north Indian men.

Authors:  S Vasisht; R Gulati; R Narang; N Srivastava; L M Srivastava; S C Manchanda; D P Agarwal
Journal:  Indian J Clin Biochem       Date:  2002-01

8.  Association between the NF-E2 Related Factor 2 Gene Polymorphism and Oxidative Stress, Anti-Oxidative Status, and Newly-Diagnosed Type 2 Diabetes Mellitus in a Chinese Population.

Authors:  Xia Wang; Hongxia Chen; Jun Liu; Yingying Ouyang; Di Wang; Wei Bao; Liegang Liu
Journal:  Int J Mol Sci       Date:  2015-07-20       Impact factor: 5.923

9.  The association between the Pro12Ala variant in the PPARγ2 gene and type 2 diabetes mellitus and obesity in a Chinese population.

Authors:  Xia Wang; Jun Liu; Yingying Ouyang; Min Fang; Hui Gao; Liegang Liu
Journal:  PLoS One       Date:  2013-08-21       Impact factor: 3.240
  9 in total

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