Literature DB >> 16711025

Detection of human parvovirus B19 nonstrutural protein DNA by nested-polymerase chain reaction in gravida serum and pregnant tissues.

Ting Shen1, Yongmei Huang, Fuyuan Qiao, Zengqing Li, Haiyi Liu.   

Abstract

A new nested-polymerase chain reaction (nested-PCR) assay was developed to detect human parvovirus B19 DNA corresponding to the nonstructural protein in clinical specimens in a routine diagnostic laboratory. The sensitivity of this highly specific assay was up to 0.005 fg of B19 DNA. Parvovirus B19 was identified in sera of 20 pregnant women with abnormal pregnant outcome. Among these 20 cases, intrauterine parvovirus infection did exist in 7 pregnant women because parvovirus B19 DNA was detected in the pregnant tissues of them such as placenta tissues, chorionic villi, amniotic fluid, fetal spleen, liver and abdominal fluids.

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Year:  2006        PMID： 16711025     DOI： 10.1007/BF02828057

Source DB:  PubMed          Journal:  J Huazhong Univ Sci Technolog Med Sci        ISSN： 1672-0733


  8 in total

1.  Prenatal diagnosis of congenital parvovirus B19 infection: value of serological and PCR techniques in maternal and fetal serum.

Authors:  D Dieck; R L Schild; M Hansmann; A M Eis-Hübinger
Journal:  Prenat Diagn       Date:  1999-12       Impact factor: 3.050

2.  A transgenic mouse model for non-immune hydrops fetalis induced by the NS1 gene of human parvovirus B19.

Authors:  Hiroshi Chisaka; Eiji Morita; Kazuko Murata; Naoto Ishii; Nobuo Yaegashi; Kunihiro Okamura; Kazuo Sugamura
Journal:  J Gen Virol       Date:  2002-02       Impact factor: 3.891

Review 3.  Pathogenesis of nonimmune hydrops fetalis caused by intrauterine B19 infection.

Authors:  N Yaegashi
Journal:  Tohoku J Exp Med       Date:  2000-02       Impact factor: 1.848

4.  Detection of human parvovirus B19 DNA by nested polymerase chain reaction.

Authors:  Y Yamakawa; H Oka; S Hori; T Arai; R Izumi
Journal:  Obstet Gynecol       Date:  1995-07       Impact factor: 7.661

5.  Frequency of human parvovirus B19 infection in intrauterine fetal death.

Authors:  T Tolfvenstam; N Papadogiannakis; O Norbeck; K Petersson; K Broliden
Journal:  Lancet       Date:  2001-05-12       Impact factor: 79.321

Review 6.  Parvovirus B19 nonstructural (NS1) protein as a transactivator of interleukin-6 synthesis: common pathway in inflammatory sequelae of human parvovirus infections?

Authors:  Leslie Ann Mitchell
Journal:  J Med Virol       Date:  2002-06       Impact factor: 2.327

7.  Seroprevalence of parvovirus B19 NS1-specific IgG in B19-infected and uninfected individuals and in infected pregnant women.

Authors:  A Hemauer; A Gigler; K Searle; K Beckenlehner; U Raab; K Broliden; H Wolf; G Enders; S Modrow
Journal:  J Med Virol       Date:  2000-01       Impact factor: 2.327

8.  Human parvovirus B19 infections: routine diagnosis by a new nested polymerase chain reaction assay.

Authors:  P Cassinotti; M Weitz; G Siegl
Journal:  J Med Virol       Date:  1993-07       Impact factor: 2.327
  8 in total

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