Literature DB >> 1671011

Immortalization of Werner syndrome and progeria fibroblasts.

H Saito1, R E Moses.   

Abstract

Human fibroblast cells from two different progeroid syndromes, Werner syndrome (WS) and progeria, were established as immortalized cell lines by transfection with plasmid DNA containing the SV40 early region. The lineage of each immortalized cell line was confirmed by VNTR analysis. Each of the immortalized cell lines maintained its original phenotype of slow growth. DNA repair ability of these cells was also studied by measuring sensitivity to killing by uv or the DNA-damaging drugs methyl methansulfonate, bleomycin, and cis-dichlorodiamine platinum. The results showed that both WS and progeria cells have normal sensitivity to these agents.

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Year:  1991        PMID： 1671011     DOI： 10.1016/0014-4827(91)90054-x

Source DB:  PubMed          Journal:  Exp Cell Res        ISSN： 0014-4827            Impact factor:   3.905


  11 in total

1.  Mutations in the consensus helicase domains of the Werner syndrome gene. Werner's Syndrome Collaborative Group.

Authors:  C E Yu; J Oshima; E M Wijsman; J Nakura; T Miki; C Piussan; S Matthews; Y H Fu; J Mulligan; G M Martin; G D Schellenberg
Journal:  Am J Hum Genet       Date:  1997-02       Impact factor: 11.025

2.  Selective blockage of the 3'-->5' exonuclease activity of WRN protein by certain oxidative modifications and bulky lesions in DNA.

Authors:  A Machwe; R Ganunis; V A Bohr; D K Orren
Journal:  Nucleic Acids Res       Date:  2000-07-15       Impact factor: 16.971

3.  Loss of Werner syndrome protein function promotes aberrant mitotic recombination.

Authors:  P R Prince; M J Emond; R J Monnat
Journal:  Genes Dev       Date:  2001-04-15       Impact factor: 11.361

4.  Reversal of the temperature-shift-induced growth restriction of a temperature-sensitive simian virus 40 T-antigen-transformed human fibroblast cell line by treatment with retinoic acid.

Authors:  Y P Tsao; S F Li; S W Kuo; J C Liu; S L Chen
Journal:  Biochem J       Date:  1996-08-01       Impact factor: 3.857

5.  Homologous recombination resolution defect in werner syndrome.

Authors:  Yannick Saintigny; Kate Makienko; Cristina Swanson; Mary J Emond; Raymond J Monnat
Journal:  Mol Cell Biol       Date:  2002-10       Impact factor: 4.272

6.  A deletion within the murine Werner syndrome helicase induces sensitivity to inhibitors of topoisomerase and loss of cellular proliferative capacity.

Authors:  M Lebel; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1998-10-27       Impact factor: 11.205

7.  Differential regulation of cyclin A, cyclin B and p21 concentrations in a growth-restricted human fibroblast cell line.

Authors:  Y P Tsao; S W Kuo; S F Li; J C Liu; S Z Lin; K Y Chen; S L Chen
Journal:  Biochem J       Date:  1995-12-15       Impact factor: 3.857

8.  The Werner syndrome helicase protein is required for cell proliferation, immortalization, and tumorigenesis in Scaffold attachment factor B1 deficient mice.

Authors:  Sophie Lachapelle; Steffi Oesterreich; Michel Lebel
Journal:  Aging (Albany NY)       Date:  2011-03       Impact factor: 5.682

9.  Modulation of telomeres in alternative lengthening of telomeres type I like human cells by the expression of werner protein and telomerase.

Authors:  Aisha Siddiqa; David Cavazos; Jeffery Chavez; Linda Long; Robert A Marciniak
Journal:  J Oncol       Date:  2012-03-28       Impact factor: 4.375

10.  Senescence induced by RECQL4 dysfunction contributes to Rothmund-Thomson syndrome features in mice.

Authors:  H Lu; E F Fang; P Sykora; T Kulikowicz; Y Zhang; K G Becker; D L Croteau; V A Bohr
Journal:  Cell Death Dis       Date:  2014-05-15       Impact factor: 8.469
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