Literature DB >> 1670930

Toxin A of Clostridium difficile binds to the human carbohydrate antigens I, X, and Y.

K D Tucker1, T D Wilkins.   

Abstract

Clostridium difficile causes pseudomembranous colitis in humans. The enterotoxin (i.e., toxin A) from this organism is believed to be responsible for the initial intestinal pathology associated with this disease. Previous work shows that this toxin binds to carbohydrates that contain Gal alpha 1-3Gal beta 1-4GlcNAc. However, this carbohydrate is not present on normal human cells. Therefore, this study was undertaken to identify potential receptors for toxin A that do exist on human intestinal epithelium. Using enzyme-linked immunosorbent assay, affinity chromatography, and altered migration in an electric field, we assayed the binding of toxin A to purified carbohydrates and glycoproteins. We found that toxin A bound to the carbohydrate antigens designated I, X, and Y. Each of these carbohydrates exist on the intestinal epithelium of humans.

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Year:  1991        PMID： 1670930      PMCID： PMC257707          DOI： 10.1128/iai.59.1.73-78.1991

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  37 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  Characterization of toxins A and B of Clostridium difficile with monoclonal antibodies.

Authors:  D M Lyerly; C J Phelps; J Toth; T D Wilkins
Journal:  Infect Immun       Date:  1986-10       Impact factor: 3.441

3.  Man, apes, and Old World monkeys differ from other mammals in the expression of alpha-galactosyl epitopes on nucleated cells.

Authors:  U Galili; S B Shohet; E Kobrin; C L Stults; B A Macher
Journal:  J Biol Chem       Date:  1988-11-25       Impact factor: 5.157

4.  Effects of Clostridium difficile toxins A and B in rabbit small and large intestine in vivo and on cultured cells in vitro.

Authors:  A A Lima; D M Lyerly; T D Wilkins; D J Innes; R L Guerrant
Journal:  Infect Immun       Date:  1988-03       Impact factor: 3.441

5.  Clostridial toxin in faeces of healthy infants.

Authors:  P J Rietra; K W Slaterus; H C Zanen; S G Meuwissen
Journal:  Lancet       Date:  1978-08-05       Impact factor: 79.321

6.  Cell surface binding site for Clostridium difficile enterotoxin: evidence for a glycoconjugate containing the sequence Gal alpha 1-3Gal beta 1-4GlcNAc.

Authors:  H C Krivan; G F Clark; D F Smith; T D Wilkins
Journal:  Infect Immun       Date:  1986-09       Impact factor: 3.441

7.  Differential effects of Clostridium difficile toxins A and B on rabbit ileum.

Authors:  G Triadafilopoulos; C Pothoulakis; M J O'Brien; J T LaMont
Journal:  Gastroenterology       Date:  1987-08       Impact factor: 22.682

8.  Expression of Lewisa, Lewisb, X, and Y blood group antigens in human colonic tumors and normal tissue and in human tumor-derived cell lines.

Authors:  J Sakamoto; K Furukawa; C Cordon-Cardo; B W Yin; W J Rettig; H F Oettgen; L J Old; K O Lloyd
Journal:  Cancer Res       Date:  1986-03       Impact factor: 12.701

9.  Asymptomatic neonatal colonisation by Clostridium difficile.

Authors:  R P Bolton; S K Tait; P R Dear; M S Losowsky
Journal:  Arch Dis Child       Date:  1984-05       Impact factor: 3.791

10.  Human natural anti-alpha-galactosyl IgG. II. The specific recognition of alpha (1----3)-linked galactose residues.

Authors:  U Galili; B A Macher; J Buehler; S B Shohet
Journal:  J Exp Med       Date:  1985-08-01       Impact factor: 14.307
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  52 in total

1.  Rabbit sucrase-isomaltase contains a functional intestinal receptor for Clostridium difficile toxin A.

Authors:  C Pothoulakis; R J Gilbert; C Cladaras; I Castagliuolo; G Semenza; Y Hitti; J S Montcrief; J Linevsky; C P Kelly; S Nikulasson; H P Desai; T D Wilkins; J T LaMont
Journal:  J Clin Invest       Date:  1996-08-01       Impact factor: 14.808

Review 2.  Clostridium difficile toxins: mechanism of action and role in disease.

Authors:  Daniel E Voth; Jimmy D Ballard
Journal:  Clin Microbiol Rev       Date:  2005-04       Impact factor: 26.132

3.  Comparative sequence analysis of the Clostridium difficile toxins A and B.

Authors:  C von Eichel-Streiber; R Laufenberg-Feldmann; S Sartingen; J Schulze; M Sauerborn
Journal:  Mol Gen Genet       Date:  1992-05

4.  Antigenic cross-reactivity and functional inhibition by antibodies to Clostridium difficile toxin A, Streptococcus mutans glucan-binding protein, and a synthetic peptide.

Authors:  B W Wren; R R Russell; S Tabaqchali
Journal:  Infect Immun       Date:  1991-09       Impact factor: 3.441

5.  Evidence for a modular structure of the homologous repetitive C-terminal carbohydrate-binding sites of Clostridium difficile toxins and Streptococcus mutans glucosyltransferases.

Authors:  C von Eichel-Streiber; M Sauerborn; H K Kuramitsu
Journal:  J Bacteriol       Date:  1992-10       Impact factor: 3.490

6.  A neutralizing antibody that blocks delivery of the enzymatic cargo of Clostridium difficile toxin TcdB into host cells.

Authors:  Heather K Kroh; Ramyavardhanee Chandrasekaran; Zhifen Zhang; Kim Rosenthal; Rob Woods; Xiaofang Jin; Andrew C Nyborg; G Jonah Rainey; Paul Warrener; Roman A Melnyk; Benjamin W Spiller; D Borden Lacy
Journal:  J Biol Chem       Date:  2017-11-27       Impact factor: 5.157

7.  Crystal structure of receptor-binding C-terminal repeats from Clostridium difficile toxin A.

Authors:  Jason G S Ho; Antonio Greco; Maja Rupnik; Kenneth K-S Ng
Journal:  Proc Natl Acad Sci U S A       Date:  2005-12-12       Impact factor: 11.205

8.  Positive regulation of Clostridium difficile toxins.

Authors:  J S Moncrief; L A Barroso; T D Wilkins
Journal:  Infect Immun       Date:  1997-03       Impact factor: 3.441

9.  Development and optimization of a novel assay to measure neutralizing antibodies against Clostridium difficile toxins.

Authors:  Jinfu Xie; Julie Zorman; Lani Indrawati; Melanie Horton; Keri Soring; Joseph M Antonello; Yuhua Zhang; Susan Secore; Matthew Miezeiewski; Su Wang; Anthony D Kanavage; Julie M Skinner; Irene Rogers; Jean-Luc Bodmer; Jon H Heinrichs
Journal:  Clin Vaccine Immunol       Date:  2013-02-06

10.  Sequencing and analysis of the gene encoding the alpha-toxin of Clostridium novyi proves its homology to toxins A and B of Clostridium difficile.

Authors:  F Hofmann; A Herrmann; E Habermann; C von Eichel-Streiber
Journal:  Mol Gen Genet       Date:  1995-06-25
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