G P Allen1, C C Breathnach. 1. Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky 40546-0099, USA.
Abstract
REASONS FOR PERFORMING STUDY: Neurological disease in horses caused by infection with certain 'paralytic' strains of equine herpesvirus-1 (EHV-1) is a potentially devastating condition the pathogenesis of which is poorly understood. Preliminary observations in both experimentally induced and naturally occurring cases of the central nervous system disease have revealed a more robust cell-associated viraemia in horses infected with paralytic isolates of EHV-1, relative to horses infected with abortigenic isolates. To investigate further this pathogenesis-relevant question, the present study was performed using a greater number of horses and a more precise method for quantification of EHV-1 DNA present in viraemic leucocytes. OBJECTIVE: To compare the magnitude and duration of leucocyte-associated viraemia in seronegative, age-matched foals following infection with paralytic vs. abortigenic isolates of EHV-1. METHODS: Peripheral blood mononuclear cells (PBMC) were collected from 20 weanling foals at 2, 4, 7, 9, 11, 14 and 21 days after intranasal inoculation with either paralytic or abortigenic isolates of EHV-1. The amount of EHV-1 DNA present in each PBMC sample was measured by real-time quantitative PCR. RESULTS: Foals inoculated with paralytic strains of EHV-1 developed both a greater magnitude and longer duration of PBMC-associated viraemia than foals inoculated with abortigenic strains of the virus. CONCLUSIONS: Both the higher magnitude and longer duration of cell-associated viraemia contribute to the risk for development of neurological signs in horses infected with paralytic strains of EHV-1. POTENTIAL RELEVANCE: Our results provide empirically derived, scientific data that contributes to a better understanding of the pathogenetic basis for the differing abilities of paralytic and abortigenic strains of EHV-1 to cause post infection central nervous system disease in the horse. The findings identify the importance of minimising the quantitative burden of viraemic leucocytes that follows exposure to the virus, by the use of effective therapeutic antiviral drugs and efficacious prophylactic vaccines that stimulate cytotoxic immune responses against EHV-1 infected cells.
REASONS FOR PERFORMING STUDY: Neurological disease in horses caused by infection with certain 'paralytic' strains of equine herpesvirus-1 (EHV-1) is a potentially devastating condition the pathogenesis of which is poorly understood. Preliminary observations in both experimentally induced and naturally occurring cases of the central nervous system disease have revealed a more robust cell-associated viraemia in horses infected with paralytic isolates of EHV-1, relative to horses infected with abortigenic isolates. To investigate further this pathogenesis-relevant question, the present study was performed using a greater number of horses and a more precise method for quantification of EHV-1 DNA present in viraemic leucocytes. OBJECTIVE: To compare the magnitude and duration of leucocyte-associated viraemia in seronegative, age-matched foals following infection with paralytic vs. abortigenic isolates of EHV-1. METHODS: Peripheral blood mononuclear cells (PBMC) were collected from 20 weanling foals at 2, 4, 7, 9, 11, 14 and 21 days after intranasal inoculation with either paralytic or abortigenic isolates of EHV-1. The amount of EHV-1 DNA present in each PBMC sample was measured by real-time quantitative PCR. RESULTS: Foals inoculated with paralytic strains of EHV-1 developed both a greater magnitude and longer duration of PBMC-associated viraemia than foals inoculated with abortigenic strains of the virus. CONCLUSIONS: Both the higher magnitude and longer duration of cell-associated viraemia contribute to the risk for development of neurological signs in horses infected with paralytic strains of EHV-1. POTENTIAL RELEVANCE: Our results provide empirically derived, scientific data that contributes to a better understanding of the pathogenetic basis for the differing abilities of paralytic and abortigenic strains of EHV-1 to cause post infection central nervous system disease in the horse. The findings identify the importance of minimising the quantitative burden of viraemic leucocytes that follows exposure to the virus, by the use of effective therapeutic antiviral drugs and efficacious prophylactic vaccines that stimulate cytotoxic immune responses against EHV-1 infected cells.
Authors: Kathryn L Smith; Yanqiu Li; Patrick Breheny; R Frank Cook; Pamela J Henney; Stephen Sells; Stéphane Pronost; Zhengchun Lu; Beate M Crossley; Peter J Timoney; Udeni B R Balasuriya Journal: J Clin Microbiol Date: 2012-04-04 Impact factor: 5.948
Authors: Aline S Hora; Paloma O Tonietti; Juliana M Guerra; Maiara C Leme; Hilda F J Pena; Paulo C Maiorka; Paulo E Brandão Journal: J Clin Microbiol Date: 2012-11-14 Impact factor: 5.948
Authors: Mathias Franz; Laura B Goodman; Gerlinde R Van de Walle; Nikolaus Osterrieder; Alex D Greenwood Journal: Viruses Date: 2017-01-10 Impact factor: 5.048
Authors: N A Bryant; G S Wilkie; C A Russell; L Compston; D Grafham; L Clissold; K McLay; L Medcalf; R Newton; A J Davison; D M Elton Journal: Transbound Emerg Dis Date: 2018-02-09 Impact factor: 5.005
Authors: Laura B Goodman; Arianna Loregian; Gillian A Perkins; Josie Nugent; Elizabeth L Buckles; Beatrice Mercorelli; Julia H Kydd; Giorgio Palù; Ken C Smith; Nikolaus Osterrieder; Nicholas Davis-Poynter Journal: PLoS Pathog Date: 2007-11 Impact factor: 6.823
Authors: Margaret M Brosnahan; Armando Damiani; Gerlinde van de Walle; Hollis Erb; Gillian A Perkins; Nikolaus Osterrieder Journal: Virus Res Date: 2009-11-05 Impact factor: 3.303