BACKGROUND: Although dendritic cells (DCs) play significant roles in intestinal immune responses, little is known regarding the direct effects of luminal foods on DC functions in the intestinal mucosa. In this study, we examined the effects of fatty acids (FAs) with various chain length on the phagocytic function, antigen presentation, and chemotaxis of intestinal DCs. METHODS: DCs obtained from the thoracic duct lymph of mesenteric lymphadenectomized rats were cultured with long [arachidonic acid (AA) or oleic acid] or medium (octanoic acid) chain FAs with interleukin-4 and granulocyte macrophage-colony stimulating factor. Tumor necrosis factor-alpha was added in the maturation group. Phagocytic function was examined by the intake of fluorescent microbeads. The expression of cell surface molecules was determined by immunocytochemistry or fluorescence-activated cell sorting (FACS). Antigen presentation ability was evaluated by coincubating keyhole limpet hemocyanin (KLH)-sensitized spleen lymphocytes and KLH-pulsed DCs. Migratory ability of DCs toward the chemokines CC chemokine ligand (CCL) 20 and CCL21 was also assessed. RESULTS: There was a maturation-induced decrease in phagocytic function, and an increased expression of major histocompatibility complex (MHC) class II molecules. Exposure of DCs to both long- and medium-chain FAs maintained phagocytic ability. The expression of MHC class II molecules was significantly suppressed only by long-chain FAs. The expression of costimulatory factors was suppressed only by AA. Long- but not medium-chain FAs suppressed the antigen presentation ability of DCs induced by maturation. Chemotactic ability of mature DCs toward CCL21 was abrogated only by long-chain FAs. CONCLUSIONS: The data suggest that intraluminal exposure to long- and medium-chain FAs may differentially modulate the immune functions of intestinal DCs.
BACKGROUND: Although dendritic cells (DCs) play significant roles in intestinal immune responses, little is known regarding the direct effects of luminal foods on DC functions in the intestinal mucosa. In this study, we examined the effects of fatty acids (FAs) with various chain length on the phagocytic function, antigen presentation, and chemotaxis of intestinal DCs. METHODS: DCs obtained from the thoracic duct lymph of mesenteric lymphadenectomized rats were cultured with long [arachidonic acid (AA) or oleic acid] or medium (octanoic acid) chain FAs with interleukin-4 and granulocyte macrophage-colony stimulating factor. Tumor necrosis factor-alpha was added in the maturation group. Phagocytic function was examined by the intake of fluorescent microbeads. The expression of cell surface molecules was determined by immunocytochemistry or fluorescence-activated cell sorting (FACS). Antigen presentation ability was evaluated by coincubating keyhole limpet hemocyanin (KLH)-sensitized spleen lymphocytes and KLH-pulsed DCs. Migratory ability of DCs toward the chemokines CC chemokine ligand (CCL) 20 and CCL21 was also assessed. RESULTS: There was a maturation-induced decrease in phagocytic function, and an increased expression of major histocompatibility complex (MHC) class II molecules. Exposure of DCs to both long- and medium-chain FAs maintained phagocytic ability. The expression of MHC class II molecules was significantly suppressed only by long-chain FAs. The expression of costimulatory factors was suppressed only by AA. Long- but not medium-chain FAs suppressed the antigen presentation ability of DCs induced by maturation. Chemotactic ability of mature DCs toward CCL21 was abrogated only by long-chain FAs. CONCLUSIONS: The data suggest that intraluminal exposure to long- and medium-chain FAs may differentially modulate the immune functions of intestinal DCs.
Authors: M Rescigno; M Urbano; B Valzasina; M Francolini; G Rotta; R Bonasio; F Granucci; J P Kraehenbuhl; P Ricciardi-Castagnoli Journal: Nat Immunol Date: 2001-04 Impact factor: 25.606
Authors: Y Tsuzuki; S Miura; I Kurose; M Suematsu; H Higuchi; T Shigematsu; H Kimura; H Serizawa; R Hokari; Y Akiba; H Yagita; K Okumura; P Tso; D N Granger; H Ishii Journal: Gastroenterology Date: 1997-03 Impact factor: 22.682
Authors: H Yoshida; S Miura; H Kishikawa; M Hirokawa; H Nakamizo; R C Nakatsumi; H Suzuki; H Saito; H Ishii Journal: J Nutr Date: 2001-11 Impact factor: 4.798
Authors: N Haicheur; E Bismuth; S Bosset; O Adotevi; G Warnier; V Lacabanne; A Regnault; C Desaymard; S Amigorena; P Ricciardi-Castagnoli; B Goud; W H Fridman; L Johannes; E Tartour Journal: J Immunol Date: 2000-09-15 Impact factor: 5.422
Authors: K Kusugami; T Ando; M Ohsuga; A Imada; M Shinoda; T Konagaya; K Ina; N Kasuga; A Fukatsu; S Ichiyama; T Nada; M Ohta Journal: J Clin Gastroenterol Date: 1997 Impact factor: 3.062
Authors: J Rodrigo Mora; Maria Rosa Bono; N Manjunath; Wolfgang Weninger; Lois L Cavanagh; Mario Rosemblatt; Ulrich H Von Andrian Journal: Nature Date: 2003-07-03 Impact factor: 49.962
Authors: Michael W Rohr; Chandrakala A Narasimhulu; Trina A Rudeski-Rohr; Sampath Parthasarathy Journal: Adv Nutr Date: 2020-01-01 Impact factor: 8.701
Authors: Stephen E Malawista; Anne de Boisfleury Chevance; Jo van Damme; Charles N Serhan Journal: Proc Natl Acad Sci U S A Date: 2008-11-07 Impact factor: 11.205