Literature DB >> 16698217

The suitable restriction enzymes for pulsed-field gel electrophoresis analysis of Bordetella pertussis.

Meng-Shiunn Lee1, Yeong-Sheng Lee, Chien-Shun Chiou.   

Abstract

We searched the restriction enzymes with rare cutting sites on the genome of Bordetella pertussis strain Tohama I using the Restriction Digest Tool software provided in the Institute for Genomic Research web site. The usefulness of 5 enzymes for pulsed-field gel electrophoresis (PFGE) analysis was evaluated with 68 B. pertussis isolates. The results indicated that AflII, DraI, SpeI, and XbaI were useful enzymes, and AflII was the best one for PFGE analysis of B. pertussis isolates.

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Year:  2006        PMID: 16698217     DOI: 10.1016/j.diagmicrobio.2006.03.017

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN: 0732-8893            Impact factor:   2.803


  2 in total

1.  Multilocus sequence typing and pulsed field gel electrophoresis of otitis media causing pathogens.

Authors:  Jonathan C Thomas; Melinda M Pettigrew
Journal:  Methods Mol Biol       Date:  2009

2.  Adaptability and persistence of the emerging pathogen Bordetella petrii.

Authors:  Adrian M Zelazny; Li Ding; Joanna B Goldberg; Lilia A Mijares; Sean Conlan; Patricia S Conville; Frida Stock; Samuel J Ballentine; Kenneth N Olivier; Elizabeth P Sampaio; Patrick R Murray; Steven M Holland
Journal:  PLoS One       Date:  2013-06-04       Impact factor: 3.240

  2 in total

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