Literature DB >> 16690030

Action of apoptotic endonuclease DNase gamma on naked DNA and chromatin substrates.

Ryushin Mizuta1, Midori Mizuta, Shinsuke Araki, Daisuke Shiokawa, Sei-ichi Tanuma, Daisuke Kitamura.   

Abstract

The internucleosomal cleavage of genomic DNA is a biochemical hallmark of apoptosis. DNase gamma, a Mg2+/Ca2+-dependent endonuclease, has been suggested to be one of the apoptotic endonucleases, but its biochemical characteristic has not been fully elucidated. Here, using recombinant DNase gamma, we showed that DNase gamma is a Mg2+/Ca2+-dependent single-stranded DNA nickase and has a high activity at low ionic strength. Under higher ionic strength, such as physiological buffer conditions, the endonuclease activity of DNase gamma is restricted, but its activity is enhanced in the presence of linker histone H1, which explains DNA cleavage at linker regions of apoptotic nuclei.

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Year:  2006        PMID： 16690030     DOI： 10.1016/j.bbrc.2006.04.107

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  5 in total

1.  Evaluation of all nonsynonymous single-nucleotide polymorphisms in the gene encoding human deoxyribonuclease I-like 1, possibly implicated in the blocking of endocytosis-mediated foreign gene transfer.

Authors:  Misuzu Ueki; Kaori Kimura-Kataoka; Junko Fujihara; Haruo Takeshita; Reiko Iida; Toshihiro Yasuda
Journal:  DNA Cell Biol       Date:  2013-12-13       Impact factor: 3.311

2.  Identification of the functional alleles of the nonsynonymous single-nucleotide polymorphisms potentially implicated in systemic lupus erythematosus in the human deoxyribonuclease I gene.

Authors:  Kaori Kimura-Kataoka; Misuzu Ueki; Haruo Takeshita; Junko Fujihara; Reiko Iida; Yasuyuki Kawai; Toshihiro Yasuda
Journal:  DNA Cell Biol       Date:  2014-05-12       Impact factor: 3.311

3.  DNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis.

Authors:  Ryushin Mizuta; Shinsuke Araki; Makoto Furukawa; Yuki Furukawa; Syota Ebara; Daisuke Shiokawa; Katsuhiko Hayashi; Sei-ichi Tanuma; Daisuke Kitamura
Journal:  PLoS One       Date:  2013-12-02       Impact factor: 3.240

Review 4.  Role of Cell-Free DNA and Deoxyribonucleases in Tumor Progression.

Authors:  Ludmila Alekseeva; Nadezhda Mironova
Journal:  Int J Mol Sci       Date:  2021-11-12       Impact factor: 5.923

5.  Detection and characterization of jagged ends of double-stranded DNA in plasma.

Authors:  Peiyong Jiang; Tingting Xie; Spencer C Ding; Ze Zhou; Suk Hang Cheng; Rebecca W Y Chan; Wing-Shan Lee; Wenlei Peng; John Wong; Vincent W S Wong; Henry L Y Chan; Stephen L Chan; Liona C Y Poon; Tak Y Leung; K C Allen Chan; Rossa W K Chiu; Y M Dennis Lo
Journal:  Genome Res       Date:  2020-08-14       Impact factor: 9.043
  5 in total

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