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Literature DB >> 16689640

Single-molecule analysis of RNA polymerase transcription.

Lu Bai¹, Thomas J Santangelo, Michelle D Wang.

Abstract

The kinetics and mechanisms of transcription are now being investigated by a repertoire of single-molecule techniques, including optical and magnetic tweezers, high-sensitivity fluorescence techniques, and atomic force microscopy. Single-molecule techniques complement traditional biochemical and crystallographic approaches, are capable of detecting the motions and dynamics of individual RNAP molecules and transcription complexes in real time, and make it possible to directly measure RNAP binding to and unwinding of template DNA, as well as RNAP translocation along the DNA during transcript synthesis.

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Year: 2006 PMID： 16689640 DOI： 10.1146/annurev.biophys.35.010406.150153

Source DB: PubMed Journal: Annu Rev Biophys Biomol Struct ISSN： 1056-8700

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Cited

37 in total

1. Promoter melting triggered by bacterial RNA polymerase occurs in three steps.

Authors: Jie Chen; Seth A Darst; D Thirumalai
Journal: Proc Natl Acad Sci U S A Date: 2010-07-01 Impact factor: 11.205

2. Distinguishing the roles of Topoisomerases I and II in relief of transcription-induced torsional stress in yeast rRNA genes.

Authors: Sarah L French; Martha L Sikes; Robert D Hontz; Yvonne N Osheim; Tashima E Lambert; Aziz El Hage; Mitchell M Smith; David Tollervey; Jeffrey S Smith; Ann L Beyer
Journal: Mol Cell Biol Date: 2010-11-22 Impact factor: 4.272

Single-molecule analysis of RNA polymerase transcription.

1. Promoter melting triggered by bacterial RNA polymerase occurs in three steps.

2. Distinguishing the roles of Topoisomerases I and II in relief of transcription-induced torsional stress in yeast rRNA genes.

3. Transchip: single-molecule detection of transcriptional elongation complexes.

Review 4. Determination of thermodynamics and kinetics of RNA reactions by force.

5. Fluctuations, pauses, and backtracking in DNA transcription.

6. RNA polymerase approaches its promoter without long-range sliding along DNA.

Review 7. Modeling stochastic kinetics of molecular machines at multiple levels: from molecules to modules.

8. Imaging and analysis of transcription on large, surface-mounted single template DNA molecules.

9. Mapping of small RNAs in the human ENCODE regions.

10. Mitigating unwanted photophysical processes for improved single-molecule fluorescence imaging.