OBJECTIVES: To evaluate the relationship between prostate stem cell antigen (PSCA) expression level in transitional cell carcinoma (TCC) of the urinary bladder and various clinicopathological features, including stage and grade; and to determine whether PSCA mRNA expression predicts disease recurrence in superficial (not muscle-invasive) TCC of the bladder. PATIENTS AND METHODS: Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) was performed on 97 TCC tissue samples and in 36 samples of normal bladder urothelium; the findings were analysed in relation to clinicopathological factors. Immunohistochemical expression was examined using light and confocal immunofluorescence microscopy to validate the RT-PCR data. RESULTS: Twenty-seven patients developed disease recurrence, while the remaining 22 had no evidence of recurrence of superficial TCC of the bladder. There was significantly higher PSCA mRNA expression in TCC than in normal urothelium samples (P = 0.008). Superficial (TaT1) tumours had significantly higher PSCA expression than muscle-invasive (> or = pT2) tumours (P < 0.001). There was no significant difference between patients with G1-2 tumours and those with G3 tumours (P = 0.109). Immunohistochemical analysis showed markedly greater PSCA expression in superficial than invasive TCC. Notably, from a multivariate analysis, the expression level of PSCA was an independent predictor of disease recurrence in superficial TCC (P = 0.012). CONCLUSIONS: These findings suggest that the PSCA expression level measured by real-time RT-PCR could be a valuable prognostic marker for tumour recurrence in superficial TCC of the bladder.
OBJECTIVES: To evaluate the relationship between prostate stem cell antigen (PSCA) expression level in transitional cell carcinoma (TCC) of the urinary bladder and various clinicopathological features, including stage and grade; and to determine whether PSCA mRNA expression predicts disease recurrence in superficial (not muscle-invasive) TCC of the bladder. PATIENTS AND METHODS: Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) was performed on 97 TCC tissue samples and in 36 samples of normal bladder urothelium; the findings were analysed in relation to clinicopathological factors. Immunohistochemical expression was examined using light and confocal immunofluorescence microscopy to validate the RT-PCR data. RESULTS: Twenty-seven patients developed disease recurrence, while the remaining 22 had no evidence of recurrence of superficial TCC of the bladder. There was significantly higher PSCA mRNA expression in TCC than in normal urothelium samples (P = 0.008). Superficial (TaT1) tumours had significantly higher PSCA expression than muscle-invasive (> or = pT2) tumours (P < 0.001). There was no significant difference between patients with G1-2 tumours and those with G3 tumours (P = 0.109). Immunohistochemical analysis showed markedly greater PSCA expression in superficial than invasive TCC. Notably, from a multivariate analysis, the expression level of PSCA was an independent predictor of disease recurrence in superficial TCC (P = 0.012). CONCLUSIONS: These findings suggest that the PSCA expression level measured by real-time RT-PCR could be a valuable prognostic marker for tumour recurrence in superficial TCC of the bladder.
Authors: Jie Gao; Peng-Tao Yang; Yan Diao; Hua-Feng Kang; Yang Zhao; Shuai Lin; Zi-Ming Wang; Meng Wang; Xi-Jing Wang; Zhi-Jun Dai Journal: Int J Clin Exp Med Date: 2015-02-15
Authors: Emma E van der Toom; Haley D Axelrod; Jean J de la Rosette; Theo M de Reijke; Kenneth J Pienta; Kenneth C Valkenburg Journal: Nat Rev Urol Date: 2019-01 Impact factor: 14.432
Authors: Geoffrey A Sonn; Andrew S Behesnilian; Ziyue Karen Jiang; Kirstin A Zettlitz; Eric J Lepin; Laurent A Bentolila; Scott M Knowles; Daniel Lawrence; Anna M Wu; Robert E Reiter Journal: Clin Cancer Res Date: 2015-10-21 Impact factor: 12.531
Authors: Xifeng Wu; Yuanqing Ye; Lambertus A Kiemeney; Patrick Sulem; Thorunn Rafnar; Giuseppe Matullo; Daniela Seminara; Teruhiko Yoshida; Norihisa Saeki; Angeline S Andrew; Colin P Dinney; Bogdan Czerniak; Zuo-feng Zhang; Anne E Kiltie; D Timothy Bishop; Paolo Vineis; Stefano Porru; Frank Buntinx; Eliane Kellen; Maurice P Zeegers; Rajiv Kumar; Peter Rudnai; Eugene Gurzau; Kvetoslava Koppova; Jose Ignacio Mayordomo; Manuel Sanchez; Berta Saez; Annika Lindblom; Petra de Verdier; Gunnar Steineck; Gordon B Mills; Alan Schned; Simonetta Guarrera; Silvia Polidoro; Shen-Chih Chang; Jie Lin; David W Chang; Katherine S Hale; Tadeusz Majewski; H Barton Grossman; Steinunn Thorlacius; Unnur Thorsteinsdottir; Katja K H Aben; J Alfred Witjes; Kari Stefansson; Christopher I Amos; Margaret R Karagas; Jian Gu Journal: Nat Genet Date: 2009-08-02 Impact factor: 38.330