| Literature DB >> 16685424 |
Jianfeng Dai1, Jiyuan Zhang, Yaqiong Sun, Qihan Wu, Liyun Sun, Chaoneng Ji, Shaohua Gu, Congjing Feng, Yi Xie, Yumin Mao.
Abstract
A novel member of human protein phosphatase 2C gene named PP2Ckappa was isolated from a human fetal brain cDNA library. The 2.0 kb cDNA encodes a 372 amino acid polypeptide with an intact protein phosphatase 2C (PP2C) catalytic domain. Reverse transcription-PCR (RT-PCR) revealed that the PP2Ckappa was widely expressed in normal human tissues. Transient transfection suggested that PP2Ckappa was localized in the nucleus in AD293 cells. Recombinant Trx-His-PP2Ckappa showed phosphatase activity toward p-nitrophenyl phosphate (pNPP), as well as oligopeptides containing phospho-threonine residues. Furthermore, the overexpression of PP2Ckappa distinctly activated the heat shock transcription factor pathway in eukaryotic cells.Entities:
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Year: 2006 PMID： 16685424
Source DB: PubMed Journal: Int J Mol Med ISSN： 1107-3756 Impact factor: 4.101