| Literature DB >> 1668400 |
R M Brindeiro1, M A Soares, A B Pacheco, O H de-Pontes, D F de-Almeida, A Tanuri.
Abstract
The expression of a native form of the foot-and-mouth disease virus RNA polymerase was obtained. Two oligonucleotides of 66 base pairs were used to rebuild the 5' end of the gene and to introduce the first methionine codon. The expression of the active polymerase in E. coli was achieved by inserting the gene before the tac promoter of the pKK223-3 plasmid.Entities:
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Year: 1991 PMID： 1668400
Source DB: PubMed Journal: Braz J Med Biol Res ISSN： 0100-879X Impact factor: 2.590