Brent Wood1. 1. Department of Laboratory Medicine, University of Washington School of Medicine, Seattle, WA 98195, USA. woodbl@u.washington.edu
Abstract
CONTEXT: The development of commercial flow cytometers capable of detecting more than 10 simultaneous fluorescent signals presents opportunities for improved diagnosis and monitoring of patients with leukemia and lymphoma. OBJECTIVE: To describe instrument and reagent characteristics necessary for successful 9-color and 10-color flow cytometry in a clinical setting. DESIGN: Systematic review of issues related to instrument settings, reagent performance, and general principles of panel construction. RESULTS: Nine-color and 10-color flow cytometry offers the possibility for increased accuracy in population identification, the ability to obtain detailed information from paucicellular specimens, improved laboratory efficiency, and the means to consistently detect abnormal populations at low levels. Careful attention to details of instrument and reagent performance allows for the development of panels suitable for screening of samples for leukemia and lymphoma in a clinical setting. CONCLUSIONS: The characteristics of this technique are particularly well suited to the analysis of leukemia and lymphoma and have the potential to revolutionize and standardize this type of analysis in the clinical laboratory.
CONTEXT: The development of commercial flow cytometers capable of detecting more than 10 simultaneous fluorescent signals presents opportunities for improved diagnosis and monitoring of patients with leukemia and lymphoma. OBJECTIVE: To describe instrument and reagent characteristics necessary for successful 9-color and 10-color flow cytometry in a clinical setting. DESIGN: Systematic review of issues related to instrument settings, reagent performance, and general principles of panel construction. RESULTS: Nine-color and 10-color flow cytometry offers the possibility for increased accuracy in population identification, the ability to obtain detailed information from paucicellular specimens, improved laboratory efficiency, and the means to consistently detect abnormal populations at low levels. Careful attention to details of instrument and reagent performance allows for the development of panels suitable for screening of samples for leukemia and lymphoma in a clinical setting. CONCLUSIONS: The characteristics of this technique are particularly well suited to the analysis of leukemia and lymphoma and have the potential to revolutionize and standardize this type of analysis in the clinical laboratory.
Authors: Roland B Walter; Ted A Gooley; Brent L Wood; Filippo Milano; Min Fang; Mohamed L Sorror; Elihu H Estey; Alexander I Salter; Emily Lansverk; Jason W Chien; Ajay K Gopal; Frederick R Appelbaum; John M Pagel Journal: J Clin Oncol Date: 2011-01-31 Impact factor: 44.544
Authors: J Gauthier; L Holmberg; D Wu; W Bensinger; A K Gopal; O Press; D Maloney; D J Green; B G Till; D Byelykh; A Shustov Journal: Bone Marrow Transplant Date: 2016-10-24 Impact factor: 5.483
Authors: David M Harris; Inbal Hazan-Haley; Kevin Coombes; Carlos Bueso-Ramos; Jie Liu; Zhiming Liu; Ping Li; Murali Ravoori; Lynne Abruzzo; Lin Han; Sheela Singh; Michael Sun; Vikas Kundra; Razelle Kurzrock; Zeev Estrov Journal: PLoS One Date: 2011-06-22 Impact factor: 3.240
Authors: Olga Sala Torra; Megan Othus; David W Williamson; Brent Wood; Ilan Kirsch; Harlan Robins; Lan Beppu; Margaret R O'Donnell; Stephen J Forman; Frederick R Appelbaum; Jerald P Radich Journal: Biol Blood Marrow Transplant Date: 2017-01-03 Impact factor: 5.742
Authors: Y Zhou; B L Wood; R B Walter; P S Becker; M-E Percival; M Bar; C Shaw; K Gardner; P Hendrie; J Abkowitz; F R Appelbaum; E Estey Journal: Leukemia Date: 2017-08-18 Impact factor: 11.528