Literature DB >> 16678992

Improved multiplex PCR method for the rapid detection of beta-lactamase genes in Escherichia coli of animal origin.

Constança Pomba1, Nuno Mendonça, Marta Costa, Deolinda Louro, Bruno Baptista, Mónica Ferreira, José Duarte Correia, Manuela Caniça.   

Abstract

We developed 2 variants, A and B, of a multiplex polymerase chain reaction method for detecting the beta-lactam resistance genes bla(TEM), bla(SHV), and bla(OXA) in 122 uropathogenic Escherichia coli animal strains. Method B yielded 98% specificity and 100% sensitivity, and method A yielded 100% and 89%, respectively. Variant B was more accurate (99%) than A (94%).

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Mesh：

Year:  2006        PMID： 16678992     DOI： 10.1016/j.diagmicrobio.2006.03.005

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  5 in total

1.  Evidence of Sharing of Klebsiella pneumoniae Strains between Healthy Companion Animals and Cohabiting Humans.

Authors:  Cátia Marques; Adriana Belas; Catarina Aboim; Patrícia Cavaco-Silva; Graça Trigueiro; Luís Telo Gama; Constança Pomba
Journal:  J Clin Microbiol       Date:  2019-05-24       Impact factor: 5.948

2.  Spread of extended-spectrum beta-lactamase CTX-M-producing escherichia coli clinical isolates in community and nosocomial environments in Portugal.

Authors:  Nuno Mendonça; Joana Leitão; Vera Manageiro; Eugénia Ferreira; Manuela Caniça
Journal:  Antimicrob Agents Chemother       Date:  2007-03-19       Impact factor: 5.191

3.  ESBL/pAmpC-Producing Escherichia coli Causing Urinary Tract Infections in Non-Related Companion Animals and Humans.

Authors:  Adriana Belas; Cátia Marques; Juliana Menezes; Luís Telo da Gama; Patrícia Cavaco-Silva; Constança Pomba
Journal:  Antibiotics (Basel)       Date:  2022-04-22

4.  Longitudinal Study Detects the Co-Carriage of ESBL and mcr-1 and -4 Genes in Escherichia coli Strains in a Portuguese Farrow-to-Finish Swine Herd.

Authors:  Tiago Lima; Laura Fernandes; Marta Matias; Ana Mateus; Eduarda Silveira; Sara Domingues; Constança Pomba; Gabriela Jorge Da Silva
Journal:  Animals (Basel)       Date:  2022-08-27       Impact factor: 3.231

5.  Biofilm and fluoroquinolone resistance of canine Escherichia coli uropathogenic isolates.

Authors:  Manuela Oliveira; Filipa Rocha Dias; Constança Pomba
Journal:  BMC Res Notes       Date:  2014-08-07
  5 in total

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