RATIONALE: Adults with cystic fibrosis (CF) are at increased risk of developing osteoporosis. During infective exacerbations, increased production of proinflammatory cytokines and markers of bone resorption have been reported. OBJECTIVE: The aim of this study is to investigate the growth and proliferation of potential osteoclast precursor cells before, during, and after intravenous antibiotic treatment of infective exacerbations in patients with CF. METHODS: Hematopoietic precursor cell growth was examined using colony formation assays using Methocult culture medium. Circulating potential osteoclast precursors were identified using four-color flow cytometry by CD14, CD33, CD34, and CD45 expression. RESULTS: At the start of an infective exacerbation increases in hematopoietic precursor colony formation (15.42 colonies/10(5) cells plated, p = 0.025), proliferation (28.5%, p < 0.001), and the numbers of circulating potential osteoclast precursors (6.5%, p < 0.001) were seen in comparison with baseline levels. These increases declined after treatment with intravenous antibiotics to a level close to baseline. CONCLUSIONS: The results demonstrate an increase in the production of potential osteoclast precursors in the peripheral blood during CF infective exacerbations. This may result in increased bone resorption and contribute to bone loss in patients with CF.
RATIONALE: Adults with cystic fibrosis (CF) are at increased risk of developing osteoporosis. During infective exacerbations, increased production of proinflammatory cytokines and markers of bone resorption have been reported. OBJECTIVE: The aim of this study is to investigate the growth and proliferation of potential osteoclast precursor cells before, during, and after intravenous antibiotic treatment of infective exacerbations in patients with CF. METHODS: Hematopoietic precursor cell growth was examined using colony formation assays using Methocult culture medium. Circulating potential osteoclast precursors were identified using four-color flow cytometry by CD14, CD33, CD34, and CD45 expression. RESULTS: At the start of an infective exacerbation increases in hematopoietic precursor colony formation (15.42 colonies/10(5) cells plated, p = 0.025), proliferation (28.5%, p < 0.001), and the numbers of circulating potential osteoclast precursors (6.5%, p < 0.001) were seen in comparison with baseline levels. These increases declined after treatment with intravenous antibiotics to a level close to baseline. CONCLUSIONS: The results demonstrate an increase in the production of potential osteoclast precursors in the peripheral blood during CF infective exacerbations. This may result in increased bone resorption and contribute to bone loss in patients with CF.
Authors: D Gensburger; S Boutroy; R Chapurlat; R Nove-Josserand; S Roche; M Rabilloud; I Durieu Journal: Osteoporos Int Date: 2016-05-11 Impact factor: 4.507
Authors: Troy D Pashuck; Sarah E Franz; Molly K Altman; Clive H Wasserfall; Mark A Atkinson; Thomas J Wronski; Terence R Flotte; Michael S Stalvey Journal: Pediatr Res Date: 2009-03 Impact factor: 3.756
Authors: Alexandra Papaioannou; Courtney C Kennedy; Andreas Freitag; George Ioannidis; John O'Neill; Colin Webber; Margaret Pui; Yves Berthiaume; Harvey R Rabin; Nigel Paterson; Alphonse Jeanneret; Elias Matouk; Josee Villeneuve; Madeline Nixon; Jonathan D Adachi Journal: Chest Date: 2008-07-18 Impact factor: 9.410
Authors: Jessica Bon; Seyed Mehdi Nouraie; Kenneth J Smith; Mark T Dransfield; Merry-Lynn McDonald; Eric A Hoffman; John D Newell; Alejandro P Comellas; Punam K Saha; Russell P Bowler; Elizabeth A Regan Journal: J Bone Miner Res Date: 2020-08-04 Impact factor: 6.741