Literature DB >> 16672538

Combination of multiplex PCR and PCR-denaturing gradient gel electrophoresis for monitoring common sourdough-associated Lactobacillus species.

Luca Settanni1, Sara Valmorri, Douwe van Sinderen, Giovanna Suzzi, Antonello Paparella, Aldo Corsetti.   

Abstract

A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs.

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Year:  2006        PMID： 16672538      PMCID： PMC1472401          DOI： 10.1128/AEM.72.5.3793-3796.2006

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  18 in total

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Journal:  J Appl Microbiol       Date:  2003       Impact factor: 3.772

Review 2.  The intestinal LABs.

Authors:  Elaine E Vaughan; Maaike C de Vries; Erwin G Zoetendal; Kaouther Ben-Amor; Antoon D L Akkermans; Willem M de Vos
Journal:  Antonie Van Leeuwenhoek       Date:  2002-08       Impact factor: 2.271

3.  A Lactobacillus helveticus-Specific DNA Probe Detects Restriction Fragment Length Polymorphisms in This Species.

Authors:  C G de Los Reyes-Gavilán; G K Limsowtin; P Tailliez; L Séchaud; J P Accolas
Journal:  Appl Environ Microbiol       Date:  1992-10       Impact factor: 4.792

4.  Improvement of fragment and primer selection for mutation detection by denaturing gradient gel electrophoresis.

Authors:  Y Wu; V M Hayes; J Osinga; I M Mulder; M W Looman; C H Buys; R M Hofstra
Journal:  Nucleic Acids Res       Date:  1998-12-01       Impact factor: 16.971

Review 5.  Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology.

Authors:  G Muyzer; K Smalla
Journal:  Antonie Van Leeuwenhoek       Date:  1998-01       Impact factor: 2.271

6.  Bifidobacterial diversity in human feces detected by genus-specific PCR and denaturing gradient gel electrophoresis.

Authors:  R M Satokari; E E Vaughan; A D Akkermans; M Saarela; W M de Vos
Journal:  Appl Environ Microbiol       Date:  2001-02       Impact factor: 4.792

7.  Detection and localization of single base changes by denaturing gradient gel electrophoresis.

Authors:  R M Myers; T Maniatis; L S Lerman
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

8.  Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA.

Authors:  Hans G H J Heilig; Erwin G Zoetendal; Elaine E Vaughan; Philippe Marteau; Antoon D L Akkermans; Willem M de Vos
Journal:  Appl Environ Microbiol       Date:  2002-01       Impact factor: 4.792

9.  Development and validation of a nested-PCR-denaturing gradient gel electrophoresis method for taxonomic characterization of bifidobacterial communities.

Authors:  R Temmerman; L Masco; T Vanhoutte; G Huys; J Swings
Journal:  Appl Environ Microbiol       Date:  2003-11       Impact factor: 4.792

10.  Characterization of bacteriocin-like inhibitory substances (BLIS) from sourdough lactic acid bacteria and evaluation of their in vitro and in situ activity.

Authors:  A Corsetti; L Settanni; D Van Sinderen
Journal:  J Appl Microbiol       Date:  2004       Impact factor: 3.772
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  4 in total

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Journal:  World J Microbiol Biotechnol       Date:  2012-03-30       Impact factor: 3.312

3.  Preliminary Investigation of Biogenic Amines in Type I Sourdoughs Produced at Home and Bakery Level.

Authors:  Giuseppe Mannino; Fortunato Cirlincione; Raimondo Gaglio; Elena Franciosi; Nicola Francesca; Giancarlo Moschetti; Alberto Asteggiano; Claudio Medana; Carla Gentile; Luca Settanni
Journal:  Toxins (Basel)       Date:  2022-04-20       Impact factor: 5.075

4.  Lactic Acid Bacteria Biota and Aroma Profile of Italian Traditional Sourdoughs From the Irpinian Area in Italy.

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Journal:  Front Microbiol       Date:  2019-07-24       Impact factor: 5.640
  4 in total

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