Distribution of components of the insulin-like growth factor system in the temporomandibular joint of the aging mouse.

The temporomandibular joint (TMJ) is a exceptional joint involved in growth as well as mastication. In adult mice, it provides a model for age related natural osteoarthritis (OA). The insulin-like growth factor (IGF-) system plays was tested because it plays important roles in cartilage biology and OA pathogenesis. Decalcified and paraffin embedded TMJs of 48 NMRI mice sacrificed in groups of three male and females each at the ages of 3, 4, 5, 7, 9, 10, 12, and 18 months were prepared for histopathology and immunohistochemistry for IGF-I and -II, IGF1 receptor and IGF binding proteins -1, -2, -3, -4, -5, and -6. Histopathological signs of OA were obvious in the TMJ of all animals older than 5 months, but did not show a clear age-related staging. Immunoreactivity for all IGF components was found in unchanged anterior and posterior regions of the condyle and in regions of advanced OA lesions. Receptor immunostaining was obvious in all ages. Most IGFBPs showed immunostaining patterns similar to IGFs. While the anterior and posterior zones of the condylar cartilage appear to be specialized judging by structure and IGF immunostaining pattern, probably due to metabolic or biomechanical peculiarities, the central portion undergoes early degeneration. In advanced OA stages, the IGF system seems to be upregulated to induce repair processes. According to their mainly inhibiting functions, IGFBPs may suppress anabolic IGF activities.