| Literature DB >> 1666893 |
S Egoramaiphol1, A Henke-Saipt, E Klapp-Kunsemüller, I Bitsch.
Abstract
A HPLC method for the determination of TPPK activity in rat liver is presented. The assay comprises an extraction of TPPK from the liver into sodium phosphate buffer, pH 7.3. TPP formed in the TPPK reaction from thiamin was converted to thiochrome and thiochrome was absorbed onto Shandon APS Hypersil. The mobile phase was acetonitrile/potassium phosphate buffer, pH 8.2, and elution was monitored spectrofluorometrically.Entities:
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Year: 1991 PMID： 1666893
Source DB: PubMed Journal: Int J Vitam Nutr Res ISSN： 0300-9831 Impact factor: 1.784