Literature DB >> 16668081

Transient Gene Expression in Protoplasts of Phaseolus vulgaris Isolated from a Cell Suspension Culture.

P Leon1, F Planckaert, V Walbot.   

Abstract

A cell suspension culture of Phaseolus vulgaris cv Negro jamapa was established. Protoplasts isolated from this culture are suitable for transient gene expression studies after DNA transfer by either electroporation or treatment with polyethylene glycol. The optimized conditions for both methods were established, taking into consideration expression and cell viability. We have investigated two features of posttranscriptional gene regulation. We demonstrate that inclusion of the RNA leader sequence, omega (Omega), derived from tobacco mosaic virus, and a dicot intron in the leader region together enhance gene expression in this legume species about 10-fold.

Entities:  

Year:  1991        PMID： 16668081      PMCID： PMC1077633          DOI： 10.1104/pp.95.3.968

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  13 in total

1.  Introns increase gene expression in cultured maize cells.

Authors:  J Callis; M Fromm; V Walbot
Journal:  Genes Dev       Date:  1987-12       Impact factor: 11.361

2.  Glutathione and fungal elicitor regulation of a plant defense gene promoter in electroporated protoplasts.

Authors:  M Dron; S D Clouse; R A Dixon; M A Lawton; C J Lamb
Journal:  Proc Natl Acad Sci U S A       Date:  1988-09       Impact factor: 11.205

3.  Sequences downstream of translation start regulate quantitative expression of two petunia rbcS genes.

Authors:  C Dean; M Favreau; D Bond-Nutter; J Bedbrook; P Dunsmuir
Journal:  Plant Cell       Date:  1989-02       Impact factor: 11.277

4.  Comparison of intron-dependent and intron-independent gene expression.

Authors:  A R Buchman; P Berg
Journal:  Mol Cell Biol       Date:  1988-10       Impact factor: 4.272

5.  RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression.

Authors:  M B Shapiro; P Senapathy
Journal:  Nucleic Acids Res       Date:  1987-09-11       Impact factor: 16.971

6.  Increased gene expression by the first intron of maize shrunken-1 locus in grass species.

Authors:  V Vasil; M Clancy; R J Ferl; I K Vasil; L C Hannah
Journal:  Plant Physiol       Date:  1989-12       Impact factor: 8.340

7.  Stable transformation of soybean by electroporation and root formation from transformed callus.

Authors:  P Christou; J E Murphy; W F Swain
Journal:  Proc Natl Acad Sci U S A       Date:  1987-06       Impact factor: 11.205

8.  Enhanced translation of chimaeric messenger RNAs containing a plant viral untranslated leader sequence.

Authors:  S A Jobling; L Gehrke
Journal:  Nature       Date:  1987 Feb 12-18       Impact factor: 49.962

9.  Firefly luciferase gene: structure and expression in mammalian cells.

Authors:  J R de Wet; K V Wood; M DeLuca; D R Helinski; S Subramani
Journal:  Mol Cell Biol       Date:  1987-02       Impact factor: 4.272

10.  Monocot and dicot pre-mRNAs are processed with different efficiencies in transgenic tobacco.

Authors:  B Keith; N H Chua
Journal:  EMBO J       Date:  1986-10       Impact factor: 11.598
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  15 in total

1.  Intron-mediated enhancement of gene expression independent of unique intron sequences and splicing.

Authors:  A B Rose; J A Beliakoff
Journal:  Plant Physiol       Date:  2000-02       Impact factor: 8.340

2.  Analysis of regulatory elements of the promoter and the 3' untranslated region of the maize Hrgp gene coding for a cell wall protein.

Authors:  M Menossi; F Rabaneda; P Puigdomènech; J A Martínez-Izquierdo
Journal:  Plant Cell Rep       Date:  2003-03-14       Impact factor: 4.570

3.  Factors influencing transient gene expression in bean (Phaseolus vulgaris L.) using an electrical particle acceleration device.

Authors:  F J Aragao; M F de Sa; M R Davey; A C Brasileiro; J C Faria; E L Rech
Journal:  Plant Cell Rep       Date:  1993-07       Impact factor: 4.570

4.  Genetic transformation of green bean callus via Agrobacterium mediated DNA transfer.

Authors:  C I Franklin; T N Trieu; B G Cassidy; R A Dixon; R S Nelson
Journal:  Plant Cell Rep       Date:  1993-01       Impact factor: 4.570

5.  Intron-mediated enhancement of gene expression in maize (Zea mays L.) and bluegrass (Poa pratensis L.).

Authors:  P Vain; K R Finer; D E Engler; R C Pratt; J J Finer
Journal:  Plant Cell Rep       Date:  1996-03       Impact factor: 4.570

6.  Effects of promoter, intron and enhancer elements on transient gene expression in sugar-cane and carrot protoplasts.

Authors:  C Rathus; R Bower; R G Birch
Journal:  Plant Mol Biol       Date:  1993-11       Impact factor: 4.076

7.  Splicing of the maize Sh1 first intron is essential for enhancement of gene expression, and a T-rich motif increases expression without affecting splicing.

Authors:  Maureen Clancy; L Curtis Hannah
Journal:  Plant Physiol       Date:  2002-10       Impact factor: 8.340

8.  Proline-rich cell wall proteins accumulate in growing regions and phloem tissue in response to water deficit in common bean seedlings.

Authors:  Marina Battaglia; Rosa M Solórzano; Magdalena Hernández; Sonia Cuéllar-Ortiz; Blanca García-Gómez; Judith Márquez; Alejandra A Covarrubias
Journal:  Planta       Date:  2006-11-16       Impact factor: 4.116

9.  Somatic embryogenesis and shoot regeneration from intact seedlings of Phaseolus acutifolius A., P. aureus (L.) Wilczek, P. coccineus L., and P. wrightii L.

Authors:  K A Malik; P K Saxena
Journal:  Plant Cell Rep       Date:  1992-04       Impact factor: 4.570

10.  The intron of Arabidopsis thaliana polyubiquitin genes is conserved in location and is a quantitative determinant of chimeric gene expression.

Authors:  S R Norris; S E Meyer; J Callis
Journal:  Plant Mol Biol       Date:  1993-03       Impact factor: 4.076
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