Literature DB >> 16667912

Separation and Partial Purification of 1,3-beta-Glucan and 1,4-beta-Glucan Synthases from Saprolegnia.

V Bulone1, V Girard, M Fèvre.   

Abstract

Enriched 1,3-beta-glucan and 1,4-beta-glucan synthase fractions from the fungus Saprolegnia were isolated by rate zonal centrifugation on glycerol gradient. Purification was improved by entrapment of the enzymes in their reaction product, i.e. microfibrillar glucans. 1,3-beta-Glucan synthases were separated from 1,4-beta-glucan synthases following resuspension of entrapped enzymes. Sodium dodecylsulfate-polyacrylamide gel electrophoresis indicated that 1,3-beta-glucan and 1,4-beta-glucan synthases may have a different polypeptide composition because they were enriched for different protein subunits (34, 48, and 50 kD for the 1,3-beta-glucan synthase and 60 kD for the 1,4-beta-glucan synthase).

Entities:  

Year:  1990        PMID： 16667912      PMCID： PMC1077448          DOI： 10.1104/pp.94.4.1748

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  12 in total

1.  Modulation of Pea Membrane beta-Glucan Synthase Activity by Calcium, Polycation, Endogenous Protease, and Protease Inhibitor.

Authors:  V Girard; G Maclachlan
Journal:  Plant Physiol       Date:  1987-09       Impact factor: 8.340

2.  Phosphatases and phosphodiesterases interfere with 1,3-beta-D-glucan synthase activity in pea epicotyl membrane preparations.

Authors:  M Saugy; V Farkas; G Maclachlan
Journal:  Eur J Biochem       Date:  1988-10-15

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Identification of the uridine 5'-diphosphoglucose (UDP-Glc) binding subunit of cellulose synthase in Acetobacter xylinum using the photoaffinity probe 5-azido-UDP-Glc.

Authors:  F C Lin; R M Brown; R R Drake; B E Haley
Journal:  J Biol Chem       Date:  1990-03-25       Impact factor: 5.157

5.  Identification of the UDP-glucose-binding polypeptide of callose synthase from Beta vulgaris L. by photoaffinity labeling with 5-azido-UDP-glucose.

Authors:  D J Frost; S M Read; R R Drake; B E Haley; B P Wasserman
Journal:  J Biol Chem       Date:  1990-02-05       Impact factor: 5.157

6.  Inhibition of Mung Bean UDP-Glucose: (1-->3)-beta-Glucan Synthase by UDP-Pyridoxal: Evidence for an Active-Site Amino Group.

Authors:  S M Read; D P Delmer
Journal:  Plant Physiol       Date:  1987-12       Impact factor: 8.340

7.  UDP-Glucose: (1-->3)-beta-Glucan Synthases from Mung Bean and Cotton: Differential Effects of Ca and Mg on Enzyme Properties and on Macromolecular Structure of the Glucan Product.

Authors:  T Hayashi; S M Read; J Bussell; M Thelen; F C Lin; R M Brown; D P Delmer
Journal:  Plant Physiol       Date:  1987-04       Impact factor: 8.340

8.  Partial Purification of Digitonin-Solubilized beta-Glucan Synthase from Red Beet Root.

Authors:  L L Eiberger; B P Wasserman
Journal:  Plant Physiol       Date:  1987-04       Impact factor: 8.340

9.  Isolation of chitin synthetase from Saccharomyces cerevisiae. Purification of an enzyme by entrapment in the reaction product.

Authors:  M S Kang; N Elango; E Mattia; J Au-Young; P W Robbins; E Cabib
Journal:  J Biol Chem       Date:  1984-12-10       Impact factor: 5.157

10.  Callose biosynthesis as a Ca2+-regulated process and possible relations to the induction of other metabolic changes.

Authors:  H Kauss
Journal:  J Cell Sci Suppl       Date:  1985
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  6 in total

Review 1.  Cellulose biosynthesis.

Authors:  D P Delmer; Y Amor
Journal:  Plant Cell       Date:  1995-07       Impact factor: 11.277

2.  Rapid Enrichment of CHAPS-Solubilized UDP-Glucose: (1,3)-beta-Glucan (Callose) Synthase from Beta vulgaris L. by Product Entrapment : Entrapment Mechanisms and Polypeptide Characterization.

Authors:  A Wu; R W Harriman; D J Frost; S M Read; B P Wasserman
Journal:  Plant Physiol       Date:  1991-10       Impact factor: 8.340

3.  Cellulose and Callose Biosynthesis in Higher Plants (I. Solubilization and Separation of (1->3)- and (1->4)-[beta]-Glucan Synthase Activities from Mung Bean).

Authors:  K Kudlicka; R M Brown
Journal:  Plant Physiol       Date:  1997-10       Impact factor: 8.340

4.  Cellulose synthesis in Phytophthora infestans is required for normal appressorium formation and successful infection of potato.

Authors:  Laura J Grenville-Briggs; Victoria L Anderson; Johanna Fugelstad; Anna O Avrova; Jamel Bouzenzana; Alison Williams; Stephan Wawra; Stephen C Whisson; Paul R J Birch; Vincent Bulone; Pieter van West
Journal:  Plant Cell       Date:  2008-03-18       Impact factor: 11.277

5.  Cell wall polysaccharide synthases are located in detergent-resistant membrane microdomains in oomycetes.

Authors:  Anne Briolay; Jamel Bouzenzana; Michel Guichardant; Christian Deshayes; Nicolas Sindt; Laurence Bessueille; Vincent Bulone
Journal:  Appl Environ Microbiol       Date:  2009-02-05       Impact factor: 4.792

6.  Identification of Growth Inhibitors of the Fish Pathogen Saprolegnia parasitica Using in silico Subtractive Proteomics, Computational Modeling, and Biochemical Validation.

Authors:  Sanjiv Kumar; Rahul Shubhra Mandal; Vincent Bulone; Vaibhav Srivastava
Journal:  Front Microbiol       Date:  2020-10-16       Impact factor: 5.640
  6 in total

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