Literature DB >> 16667844

Partial Purification and Characterization of the Gibberellin A(20) 3beta-Hydroxylase from Seeds of Phaseolus vulgaris.

V A Smith1, P Gaskin, J Macmillan.   

Abstract

The GA(20) 3beta-hydroxylase present in immature seeds of Phaseolus vulgaris has been partially purified and characterized. The physical characteristics of the enzyme are similar to those of the GA 2beta-hydroxylases present in mature and immature seeds of Pisum sativum. It is acid-labile, hydrophobic, and of M(r) 45,000. The enzyme catalyzes the synthesis of GA(1), GA(5), and GA(29) from GA(20). Activity is dependent upon the presence of Fe(2+), ascorbate, 2-oxoglutarate, and oxygen. 2-Oxoglutarate does not function as a cosubstrate; in the presence of the enzyme, succinate is not a reaction product.

Entities:  

Year:  1990        PMID： 16667844      PMCID： PMC1077389          DOI： 10.1104/pp.94.3.1390

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  14 in total

1.  Regulation of newly evolved enzymes. II. The ebg repressor.

Authors:  B G Gall; D L Hartl
Journal:  Genetics       Date:  1975-11       Impact factor: 4.562

2.  The role of ascorbate in the prolyl hydroxylase reaction.

Authors:  R Myllylä; E R Kuutti-Savolainen; K I Kivirikko
Journal:  Biochem Biophys Res Commun       Date:  1978-07-28       Impact factor: 3.575

3.  An immunological approach to gibberellin purification and quantification.

Authors:  V A Smith; J Macmillan
Journal:  Plant Physiol       Date:  1989-07       Impact factor: 8.340

4.  Mechanism of the prolyl hydroxylase reaction. 1. Role of co-substrates.

Authors:  L Tuderman; R Myllylä; K I Kivirikko
Journal:  Eur J Biochem       Date:  1977-11-01

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  18-O studies of the 2-ketoglutarate-dependent sequential oxygenation of thymine to 5-carboxyuracil.

Authors:  E Holme; G Lindstedt; S Lindstedt; M Tofft
Journal:  J Biol Chem       Date:  1971-05-25       Impact factor: 5.157

7.  Simultaneous incorporation of 18 O into succinate and hydroxyproline catalyzed by collagen proline hydroxylase.

Authors:  G J Cardinale; R E Rhoads; S Udenfriend
Journal:  Biochem Biophys Res Commun       Date:  1971-05-07       Impact factor: 3.575

8.  Properties of prolyl 4-hydroxylase containing firmly-bound iron.

Authors:  J J Nietfeld; A Kemp
Journal:  Biochim Biophys Acta       Date:  1980-06-13

9.  Evidence for Phytochrome Regulation of Gibberellin A(20) 3beta-Hydroxylation in Shoots of Dwarf (lele) Pisum sativum L.

Authors:  B R Campell; B A Bonner
Journal:  Plant Physiol       Date:  1986-12       Impact factor: 8.340

10.  Qualitative and Quantitative Analyses of Gibberellins in Vegetative Shoots of Normal, dwarf-1, dwarf-2, dwarf-3, and dwarf-5 Seedlings of Zea mays L.

Authors:  S Fujioka; H Yamane; C R Spray; P Gaskin; J Macmillan; B O Phinney; N Takahashi
Journal:  Plant Physiol       Date:  1988-12       Impact factor: 8.340
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  9 in total

1.  Cloning and functional analysis of two gibberellin 3 beta -hydroxylase genes that are differently expressed during the growth of rice.

Authors:  H Itoh; M Ueguchi-Tanaka; N Sentoku; H Kitano; M Matsuoka; M Kobayashi
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-03       Impact factor: 11.205

2.  Cloning and expression of a gibberellin 2 beta,3 beta-hydroxylase cDNA from pumpkin endosperm.

Authors:  T Lange; S Robatzek; A Frisse
Journal:  Plant Cell       Date:  1997-08       Impact factor: 11.277

3.  Purification and partial amino-acid sequence of gibberellin 20-oxidase from Cucurbita maxima L. endosperm.

Authors:  T Lange
Journal:  Planta       Date:  1994       Impact factor: 4.116

4.  The Distribution of Gibberellins in Vegetative Tissues of Pisum sativum L. : I. Biological and Biochemical Consequences of the le Mutation.

Authors:  V A Smith; C J Knatt; P Gaskin; J B Reid
Journal:  Plant Physiol       Date:  1992-06       Impact factor: 8.340

5.  Function and substrate specificity of the gibberellin 3beta-hydroxylase encoded by the Arabidopsis GA4 gene.

Authors:  J Williams; A L Phillips; P Gaskin; P Hedden
Journal:  Plant Physiol       Date:  1998-06       Impact factor: 8.340

6.  Purification and characterization of 1-aminocyclopropane-1-carboxylate oxidase from apple fruit.

Authors:  J G Dong; J C Fernández-Maculet; S F Yang
Journal:  Proc Natl Acad Sci U S A       Date:  1992-10-15       Impact factor: 11.205

7.  Purification, properties and partial amino-acid sequence of 1-aminocyclopropane-1-carboxylic acid oxidase from apple fruits.

Authors:  E Dupille; C Rombaldi; J M Lelièvre; J C Cleyet-Marel; J C Pech; A Latché
Journal:  Planta       Date:  1993       Impact factor: 4.116

8.  Function and transcript analysis of gibberellin-biosynthetic enzymes in wheat.

Authors:  Nigel E J Appleford; Daniel J Evans; John R Lenton; Paul Gaskin; Stephen J Croker; Katrien M Devos; Andrew L Phillips; Peter Hedden
Journal:  Planta       Date:  2005-09-14       Impact factor: 4.116

Review 9.  2-Oxoglutarate: linking TCA cycle function with amino acid, glucosinolate, flavonoid, alkaloid, and gibberellin biosynthesis.

Authors:  Wagner L Araújo; Auxiliadora O Martins; Alisdair R Fernie; Takayuki Tohge
Journal:  Front Plant Sci       Date:  2014-10-15       Impact factor: 5.753
  9 in total

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