Literature DB >> 16666995

A New Reversed Phase-HPLC Method Resolving All Major Higher Plant Photosynthetic Pigments.

J de Las Rivas1, A Abadía, J Abadía.   

Abstract

A new reversed phase-high performance liquid chromatography method has been developed to analyze the full complement of higher plant photosynthetic pigments (cis-neoxanthin, neoxanthin, violaxanthin, taraxanthin, anteraxanthin, lutein, zeaxanthin, cis-lutein, chlorophyll b, chlorophyll a, alpha- and beta-carotene). The separation is carried out on a C(18) column in about 10 minutes, using a single high-pressure pump and three different mobile phases in three isocratic steps. This method introduces a major improvement in higher plant photosynthetic pigment analysis, resolving in only 10 minutes all photosynthetic pigments while achieving good separation of lutein from its isomer zeaxanthin. Zeaxanthin is involved in the xanthophyll cycle, which recently has been proposed to play a significant role in the protection of the photosynthetic apparatus from photoinhibitory conditions (Demmig et al. [1987] Plant Physiol 84: 218-224).

Entities:  

Year:  1989        PMID： 16666995      PMCID： PMC1061973          DOI： 10.1104/pp.91.1.190

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  4 in total

1.  COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.

Authors:  D I Arnon
Journal:  Plant Physiol       Date:  1949-01       Impact factor: 8.340

2.  Assay of carotenoids.

Authors:  N I Krinsky; S Welankiwar
Journal:  Methods Enzymol       Date:  1984       Impact factor: 1.600

3.  High-performance liquid chromatography of carotenoids.

Authors:  M Ruddat; O H Will
Journal:  Methods Enzymol       Date:  1985       Impact factor: 1.600

4.  Photoinhibition and zeaxanthin formation in intact leaves : a possible role of the xanthophyll cycle in the dissipation of excess light energy.

Authors:  B Demmig; K Winter; A Krüger; F C Czygan
Journal:  Plant Physiol       Date:  1987-06       Impact factor: 8.340
  4 in total
  18 in total

1.  Iron deficiency decreases the Fe(III)-chelate reducing activity of leaf protoplasts.

Authors:  E B González-Vallejo; F Morales; L Cistué; A Abadía; J Abadía
Journal:  Plant Physiol       Date:  2000-02       Impact factor: 8.340

2.  Iron deficiency interrupts energy transfer from a disconnected part of the antenna to the rest of Photosystem II.

Authors:  F Morales; N Moise; R Quílez; A Abadía; J Abadía; I Moya
Journal:  Photosynth Res       Date:  2001       Impact factor: 3.573

3.  UV-B exposure enhances senescence of wheat leaves: Modulation by photosynthetically active radiation.

Authors:  M K Pradhan; P N Joshi; J S Nair; N K Ramaswamy; R K Iyer; B Biswal; U C Biswal
Journal:  Radiat Environ Biophys       Date:  2006-07-19       Impact factor: 1.925

4.  Presence of 'PSI free' LHCI and monomeric LHCII and subsequent effects on fluorescence characteristics in lincomycin treated maize.

Authors:  László Gáspár; Eva Sárvári; Fermín Morales; Zoltán Szigeti
Journal:  Planta       Date:  2005-11-15       Impact factor: 4.116

5.  Long-lived primary radical pair state detected by time-resolved fluorescence and absorption spectroscopy in an isolated Photosystem two core.

Authors:  J De Las Rivas; B Crystall; P J Booth; J R Durrant; S Ozer; G Porter; D R Klug; J Barber
Journal:  Photosynth Res       Date:  1992-12       Impact factor: 3.573

6.  Photosystem II efficiency and mechanisms of energy dissipation in iron-deficient, field-grown pear trees (Pyrus communis L.).

Authors:  F Morales; R Belkhodja; A Abadía; J Abadía
Journal:  Photosynth Res       Date:  2000       Impact factor: 3.573

7.  Chlorophyll Fluorescence and Photon Yield of Oxygen Evolution in Iron-Deficient Sugar Beet (Beta vulgaris L.) Leaves.

Authors:  F Morales; A Abadía; J Abadía
Journal:  Plant Physiol       Date:  1991-11       Impact factor: 8.340

8.  Characterization of the Xanthophyll Cycle and Other Photosynthetic Pigment Changes Induced by Iron Deficiency in Sugar Beet (Beta vulgaris L.).

Authors:  F Morales; A Abadía; J Abadía
Journal:  Plant Physiol       Date:  1990-10       Impact factor: 8.340

9.  Fe Resupply to Fe-deficient Sugar Beet Plants Leads to Rapid Changes in the Violaxanthin Cycle and other Photosynthetic Characteristics without Significant de novo Chlorophyll Synthesis.

Authors:  Ajmi Larbi; Anunciación Abadía; Fermín Morales; Javier Abadía
Journal:  Photosynth Res       Date:  2004       Impact factor: 3.573

10.  Dehydration rate and time of desiccation affect recovery of the lichen alga [corrected] Trebouxia erici: alternative and classical protective mechanisms.

Authors:  Francisco Gasulla; Pedro Gómez de Nova; Alberto Esteban-Carrasco; José M Zapata; Eva Barreno; Alfredo Guéra
Journal:  Planta       Date:  2009-09-23       Impact factor: 4.116
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