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Literature DB >> 16666803

Vacuolar Acid hydrolysis as a physiological mechanism for sucrose breakdown.

E Echeverria¹, J K Burns.
1. University of Florida, IFAS.

Abstract

Sucrose breakdown in mature acidic ;Persian' limes (Citrus aurantifolia [Christm.] Swing.) occurred at a rate of 30.6 picomoles per milliliter per day during 9 weeks storage at 15 degrees C. Neither enzyme of sucrose catabolism (sucrose synthase or acid/alkaline invertase) was present in extracts of mature storage tissue. The average vacuolar pH, estimated by direct measurement of sap from isolated vacuoles and by the methylamine method, was about 2.0 to 2.2. In vitro acid hydrolysis of sucrose at physiological concentrations in a buffered solution (pH 2.2) occurred at identical rates as in matured limes. The results indicate that sucrose breakdown in stored mature acidic limes occurs by acid hydrolysis.

Entities: Chemical Species

Year: 1989 PMID： 16666803 PMCID： PMC1061756 DOI： 10.1104/pp.90.2.530

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

8 in total

1. The location of acid invertase activity and sucrose in the vacuoles of storage roots of beetroot (Beta vulgaris).

Authors: R A Leigh; T Rees; W A Fuller; J Banfield
Journal: Biochem J Date: 1979-03-15 Impact factor: 3.857

2. Studies on carbohydrate metabolism in fruit tissues of Citrus Acida. IV. Studies on glycolysis and ascorbic acid metabolism in different parts of the fruits of Citrus acida and changes in the same during maturation.

Authors: L J Parekh; V J Shah
Journal: Enzymologia Date: 1971-07-31

3. Determination of pH in chloroplasts. I. Distribution of ( 14 C) methylamine.

Authors: H Rottenberg; T Grunwald; M Avron
Journal: Eur J Biochem Date: 1972-01-31

4. Studies on carbohydrate metabolism in fruit tissues of Citrus acida. I. Detection of the enzymes of the glycolytic cycle in the fruit tissues of Citrus acida.

Authors: L J Parekh; K K Sakariah; V J Shah
Journal: Enzymologia Date: 1970-01-30

5. Studies on carbohydrate metabolism in fruit tissues of Citrus acida. II. Studies on glycolytic enzymes and glycolytic intermediates in the fruit tissues of Citrus acida during the development of the fruit.

Authors: L J Parekh; K K Sakariah; V J Shah
Journal: Enzymologia Date: 1970-01-30

6. Effect of sulfhydryl reagents on glucose determination by the glucose oxidase method.

Authors: D M Kilburn; P M Taylor
Journal: Anal Biochem Date: 1969-03 Impact factor: 3.365

7. A novel sucrose synthase pathway for sucrose degradation in cultured sycamore cells.

Authors: S C Huber; T Akazawa
Journal: Plant Physiol Date: 1986-08 Impact factor: 8.340

8. Membrane-bound ATPase of intact vacuoles and tonoplasts isolated from mature plant tissue.

Authors: W Lin; G J Wagner; H W Siegelman; G Hind
Journal: Biochim Biophys Acta Date: 1977-02-14

8 in total

1. Cloning and molecular analysis of cDNAs encoding three sucrose phosphate synthase isoforms from a citrus fruit (Citrus unshiu Marc.).

Authors: A Komatsu; Y Takanokura; M Omura; T Akihama
Journal: Mol Gen Genet Date: 1996-09-13

Vacuolar Acid hydrolysis as a physiological mechanism for sucrose breakdown.

1. The location of acid invertase activity and sucrose in the vacuoles of storage roots of beetroot (Beta vulgaris).

2. Studies on carbohydrate metabolism in fruit tissues of Citrus Acida. IV. Studies on glycolysis and ascorbic acid metabolism in different parts of the fruits of Citrus acida and changes in the same during maturation.

3. Determination of pH in chloroplasts. I. Distribution of ( 14 C) methylamine.

4. Studies on carbohydrate metabolism in fruit tissues of Citrus acida. I. Detection of the enzymes of the glycolytic cycle in the fruit tissues of Citrus acida.

5. Studies on carbohydrate metabolism in fruit tissues of Citrus acida. II. Studies on glycolytic enzymes and glycolytic intermediates in the fruit tissues of Citrus acida during the development of the fruit.

6. Effect of sulfhydryl reagents on glucose determination by the glucose oxidase method.

7. A novel sucrose synthase pathway for sucrose degradation in cultured sycamore cells.

8. Membrane-bound ATPase of intact vacuoles and tonoplasts isolated from mature plant tissue.

1. Cloning and molecular analysis of cDNAs encoding three sucrose phosphate synthase isoforms from a citrus fruit (Citrus unshiu Marc.).

2. Developmental Transition from Enzymatic to Acid Hydrolysis of Sucrose in Acid Limes (Citrus aurantifolia).

3. Postphloem, nonvascular transfer in citrus: kinetics, metabolism, and sugar gradients.

4. Sucrose-metabolizing enzymes in transport tissues and adjacent sink structures in developing citrus fruit.

5. Vacuolar citrate/H+ symporter of citrus juice cells.

6. Two tonoplast MATE proteins function as turgor-regulating chloride channels in Arabidopsis.

7. Hyperacidification of Citrus fruits by a vacuolar proton-pumping P-ATPase complex.

Review 8. Mechanisms and regulation of organic acid accumulation in plant vacuoles.