Literature DB >> 16666800

Procedures for the Generation of Mature Chlamydomonas reinhardtii Zygotes for Molecular and Biochemical Analyses.

D Wegener1, U Treier, C F Beck.   

Abstract

Zygotes represent an important stage in the sexual cycle of the unicellular green alga Chlamydomonas reinhardtii. To study zygote germination at a molecular level, a protocol was elaborated for the generation of zygotes in large quantities and a method was developed for the extraction from zygotes of RNA that could be translated in vitro.

Entities:  

Year:  1989        PMID： 16666800      PMCID： PMC1061753          DOI： 10.1104/pp.90.2.512

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  12 in total

1.  ISOLATION AND CHARACTERIZATION OF RIBOSOMAL RIBONUCLEIC ACID.

Authors:  K S KIRBY
Journal:  Biochem J       Date:  1965-07       Impact factor: 3.857

2.  The genetics and cytology of Chlamydomonas.

Authors:  R P LEVINE; W T EBERSOLD
Journal:  Annu Rev Microbiol       Date:  1960       Impact factor: 15.500

3.  Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi.

Authors:  D S Gorman; R P Levine
Journal:  Proc Natl Acad Sci U S A       Date:  1965-12       Impact factor: 11.205

4.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors:  W M Bonner; R A Laskey
Journal:  Eur J Biochem       Date:  1974-07-01

5.  Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.

Authors:  R D Palmiter
Journal:  Biochemistry       Date:  1974-08-13       Impact factor: 3.162

6.  An efficient mRNA-dependent translation system from reticulocyte lysates.

Authors:  H R Pelham; R J Jackson
Journal:  Eur J Biochem       Date:  1976-08-01

7.  Preparation and use of nuclease-treated rabbit reticulocyte lysates for the translation of eukaryotic messenger RNA.

Authors:  R J Jackson; T Hunt
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

8.  Methods for the preparation of messenger RNA.

Authors:  P M Lizardi
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

9.  Gametic differentiation in Chlamydomonas reinhardtii. I. Production of gametes and their fine structure.

Authors:  N C Martin; U W Goodenough
Journal:  J Cell Biol       Date:  1975-12       Impact factor: 10.539

10.  Nutritional control of sexuality in Chlamydomonas reinhardi.

Authors:  R SAGER; S GRANICK
Journal:  J Gen Physiol       Date:  1954-07-20       Impact factor: 4.086
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  8 in total

1.  Action Spectrum for the Light-Dependent Step in Gametic Differentiation of Chlamydomonas reinhardtii.

Authors:  H Weissig; C F Beck
Journal:  Plant Physiol       Date:  1991-09       Impact factor: 8.340

2.  Identification of novel genes specifically expressed in Chlamydomonas reinhardtii zygotes.

Authors:  D Wegener; C F Beck
Journal:  Plant Mol Biol       Date:  1991-06       Impact factor: 4.076

3.  Gametic Differentiation of Chlamydomonas reinhardtii: Control by Nitrogen and Light.

Authors:  C F Beck; A Acker
Journal:  Plant Physiol       Date:  1992-03       Impact factor: 8.340

4.  Genes expressed during sexual differentiation of Chlamydomonas reinhardtii.

Authors:  E D von Gromoff; C F Beck
Journal:  Mol Gen Genet       Date:  1993-11

5.  An Animal-Like Cryptochrome Controls the Chlamydomonas Sexual Cycle.

Authors:  Yong Zou; Sandra Wenzel; Nico Müller; Katja Prager; Elke-Martina Jung; Erika Kothe; Tilman Kottke; Maria Mittag
Journal:  Plant Physiol       Date:  2017-05-03       Impact factor: 8.340

6.  A complex system of small RNAs in the unicellular green alga Chlamydomonas reinhardtii.

Authors:  Tao Zhao; Guanglin Li; Shijun Mi; Shan Li; Gregory J Hannon; Xiu-Jie Wang; Yijun Qi
Journal:  Genes Dev       Date:  2007-04-30       Impact factor: 11.361

7.  Functional analysis and regulation of the malate synthase from Chlamydomonas reinhardtii.

Authors:  J Nogales; M I Guijo; A Quesada; F Merchán
Journal:  Planta       Date:  2004-02-27       Impact factor: 4.116

8.  Comparative genomics in Chlamydomonas and Plasmodium identifies an ancient nuclear envelope protein family essential for sexual reproduction in protists, fungi, plants, and vertebrates.

Authors:  Jue Ning; Thomas D Otto; Claudia Pfander; Frank Schwach; Mathieu Brochet; Ellen Bushell; David Goulding; Mandy Sanders; Paul A Lefebvre; Jimin Pei; Nick V Grishin; Gary Vanderlaan; Oliver Billker; William J Snell
Journal:  Genes Dev       Date:  2013-05-15       Impact factor: 11.361
  8 in total

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