Literature DB >> 16666543

Immunogold localization of photosynthetic fructose-1,6-bisphosphatase in pea leaf tissue.

R Hermoso1, M R de Felipe, A Vivó, A Chueca, J J Lázaro, J L Gorge.   

Abstract

An enriched IgG serum fraction obtained from rabbits immunized against pea chloroplast fructose-1,6-bisphosphatase (FBPase) was used, coupled to colloidal gold (15 nanometer particles) goat anti-rabbit IgG, to analyze by electron microscopy the location of photosynthetic FBPase in pea (Pisum sativum L.) leaf ultrathin sections. In accordance with earlier biochemical studies on distribution of FBPase activity, the enzyme was visualized both in the stromal space and bound to the chloroplast membranes. Some gold particles also appear in the cytoplasm, which can be related to the presence in the cytosol of a high molecular weight precursor of this nuclear coded enzyme.

Entities:  

Year:  1989        PMID： 16666543      PMCID： PMC1055847          DOI： 10.1104/pp.89.1.381

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  17 in total

1.  Light-dependent changes of the Mg2+ concentration in the stroma in relation to the Mg2+ dependency of CO2 fixation in intact chloroplasts.

Authors:  A R Portis; H W Heldt
Journal:  Biochim Biophys Acta       Date:  1976-12-06

2.  Amino acid sequence similarity between spinach chloroplast and mammalian gluconeogenic fructose-1,6-bisphosphatase.

Authors:  P B Harrsch; Y Kim; J L Fox; F Marcus
Journal:  Biochem Biophys Res Commun       Date:  1985-12-17       Impact factor: 3.575

3.  Chloroplast ribulose-5-phosphate kinase: light-mediated activation, and detection of both soluble and membrane-associated activity.

Authors:  K H Fischer; E Latzko
Journal:  Biochem Biophys Res Commun       Date:  1979-07-12       Impact factor: 3.575

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Authors:  A Vivo; M R Felipe
Journal:  Cell Mol Biol       Date:  1986       Impact factor: 1.770

5.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

Review 7.  The transport of proteins into chloroplasts.

Authors:  G W Schmidt; M L Mishkind
Journal:  Annu Rev Biochem       Date:  1986       Impact factor: 23.643

8.  Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue.

Authors:  J Roth; M Bendayan; E Carlemalm; W Villiger; M Garavito
Journal:  J Histochem Cytochem       Date:  1981-05       Impact factor: 2.479

9.  Optimal conditions for post-translational uptake of proteins by isolated chloroplasts. In vitro synthesis and transport of plastocyanin, ferredoxin-NADP+ oxidoreductase, and fructose-1,6-bisphosphatase.

Authors:  A R Grossman; S G Bartlett; G W Schmidt; J E Mullet; N H Chua
Journal:  J Biol Chem       Date:  1982-02-10       Impact factor: 5.157

10.  Thioredoxin/fructose-1,6-bisphosphatase affinity in the enzyme activation by the ferredoxin-thioredoxin system.

Authors:  A Pla; J Lopez-Gorge
Journal:  Biochim Biophys Acta       Date:  1981-06-12
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  8 in total

1.  Calvin cycle multienzyme complexes are bound to chloroplast thylakoid membranes of higher plants in situ.

Authors:  K H Suss; C Arkona; R Manteuffel; K Adler
Journal:  Proc Natl Acad Sci U S A       Date:  1993-06-15       Impact factor: 11.205

2.  Chloroplast fructose-1,6-bisphosphatase: structure and function.

Authors:  Ana Chueca; Mariam Sahrawy; Eduardo A Pagano; Julio López Gorgé
Journal:  Photosynth Res       Date:  2002       Impact factor: 3.573

3.  Purification and properties of pea (Pisum sativum L.) thioredoxin f, a plant thioredoxin with unique features in the activation of chloroplast fructose-1,6-bisphosphatase.

Authors:  F E Prado; J J Lázaro; R Hermoso; A Chueca; J L Gorgé
Journal:  Planta       Date:  1992-10       Impact factor: 4.116

4.  Cellular localization of water soluble, allergenic proteins in rye-grass (Lolium perenne) pollen using monoclonal and specific IgE antibodies with immunogold probes.

Authors:  I A Staff; P E Taylor; P Smith; M B Singh; R B Knox
Journal:  Histochem J       Date:  1990-05

5.  Biochemical characterization of stromal and thylakoid-bound isoforms of isoprene synthase in willow leaves

Authors: 
Journal:  Plant Physiol       Date:  1998-03       Impact factor: 8.340

6.  Expression and subcellular location of the tetrapyrrole synthesis enzyme porphobilinogen deaminase in light-grown Euglena gracilis and three nonchlorophyllous cell lines.

Authors:  L S Shashidhara; A G Smith
Journal:  Proc Natl Acad Sci U S A       Date:  1991-01-01       Impact factor: 11.205

7.  Cloning, structure and expression of a pea cDNA clone coding for a photosynthetic fructose-1,6-bisphosphatase with some features different from those of the leaf chloroplast enzyme.

Authors:  J L Carrasco; A Chueca; F E Prado; R Hermoso; J J Lázaro; J L Ramos; M Sahrawy; J López Gorgé
Journal:  Planta       Date:  1994       Impact factor: 4.116

8.  Effect of leaf position on levels of the chloroplast and cytosolic fructose bisphosphatase isozymes in the pea leaf nucleus.

Authors:  L E Anderson; J T Gibbons
Journal:  Protoplasma       Date:  2007-03-20       Impact factor: 3.186
  8 in total

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