Literature DB >> 16666506

Preparation of chlamydomonas chloroplasts for the in vitro import of polypeptide precursors.

M Goldschmidt-Clermont1, P Malnoë, J D Rochaix.   

Abstract

To study the import of polypeptide precursors we have adapted and compared two procedures for the isolation of competent chloroplasts from the green unicellular alga, Chlamydomonas reinhardtii: silicasol gradient centrifugation and elutriation. The chloroplasts actively import the precursor of the small subunit of ribulose bisphosphate carboxylase-oxygenase in vitro.

Entities:  

Year:  1989        PMID: 16666506      PMCID: PMC1055791          DOI: 10.1104/pp.89.1.15

Source DB:  PubMed          Journal:  Plant Physiol        ISSN: 0032-0889            Impact factor:   8.340


  15 in total

1.  Post-translational transport into intact chloroplasts of a precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase.

Authors:  N H Chua; G W Schmidt
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

2.  Evidence that a Chloroplast Surface Protein Is Associated with a Specific Binding Site for the Precursor to the Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase.

Authors:  K L Cornwell; K Keegstra
Journal:  Plant Physiol       Date:  1987-11       Impact factor: 8.340

3.  Successive translocation into and out of the mitochondrial matrix: targeting of proteins to the intermembrane space by a bipartite signal peptide.

Authors:  F U Hartl; J Ostermann; B Guiard; W Neupert
Journal:  Cell       Date:  1987-12-24       Impact factor: 41.582

4.  Identification of a receptor for protein import into chloroplasts and its localization to envelope contact zones.

Authors:  D Pain; Y S Kanwar; G Blobel
Journal:  Nature       Date:  1988-01-21       Impact factor: 49.962

5.  The role of the transit peptide in the routing of precursors toward different chloroplast compartments.

Authors:  S Smeekens; C Bauerle; J Hageman; K Keegstra; P Weisbeek
Journal:  Cell       Date:  1986-08-01       Impact factor: 41.582

Review 6.  The transport of proteins into chloroplasts.

Authors:  G W Schmidt; M L Mishkind
Journal:  Annu Rev Biochem       Date:  1986       Impact factor: 23.643

7.  Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii.

Authors:  M Goldschmidt-Clermont; M Rahire
Journal:  J Mol Biol       Date:  1986-10-05       Impact factor: 5.469

8.  The cleavable pre-sequence of an imported chloroplast protein directs attached polypeptides into yeast mitochondria.

Authors:  E C Hurt; N Soltanifar; M Goldschmidt-Clermont; J D Rochaix; G Schatz
Journal:  EMBO J       Date:  1986-06       Impact factor: 11.598

9.  Transit peptides of nuclear-encoded chloroplast proteins share a common amino acid framework.

Authors:  G A Karlin-Neumann; E M Tobin
Journal:  EMBO J       Date:  1986-01       Impact factor: 11.598

10.  Functional determinants in transit sequences: import and partial maturation by vascular plant chloroplasts of the ribulose-1,5-bisphosphate carboxylase small subunit of Chlamydomonas.

Authors:  M L Mishkind; S R Wessler; G W Schmidt
Journal:  J Cell Biol       Date:  1985-01       Impact factor: 10.539

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  13 in total

1.  Translation of chloroplast psbA mRNA is modulated in the light by counteracting oxidizing and reducing activities.

Authors:  T Trebitsh; A Levitan; A Sofer; A Danon
Journal:  Mol Cell Biol       Date:  2000-02       Impact factor: 4.272

2.  Translation of chloroplast psbA mRNA is regulated by signals initiated by both photosystems II and I.

Authors:  T Trebitsh; A Danon
Journal:  Proc Natl Acad Sci U S A       Date:  2001-10-02       Impact factor: 11.205

3.  Characterization of an internal type-II NADH dehydrogenase from Chlamydomonas reinhardtii mitochondria.

Authors:  Renaud Lecler; Hélène Vigeolas; Barbara Emonds-Alt; Pierre Cardol; Claire Remacle
Journal:  Curr Genet       Date:  2012-07-20       Impact factor: 3.886

4.  An mt(+) gamete-specific nuclease that targets mt(-) chloroplasts during sexual reproduction in C. reinhardtii.

Authors:  Yoshiki Nishimura; Osami Misumi; Ko Kato; Noriko Inada; Tetsuya Higashiyama; Yu Momoyama; Tsuneyoshi Kuroiwa
Journal:  Genes Dev       Date:  2002-05-01       Impact factor: 11.361

5.  The 5' leader of a chloroplast mRNA mediates the translational requirements for two nucleus-encoded functions in Chlamydomonas reinhardtii.

Authors:  W Zerges; J D Rochaix
Journal:  Mol Cell Biol       Date:  1994-08       Impact factor: 4.272

6.  Chlamydomonas reinhardtii encodes a single sigma70-like factor which likely functions in chloroplast transcription.

Authors:  Alexandra-V Bohne; Vered Irihimovitch; Andreas Weihe; David B Stern
Journal:  Curr Genet       Date:  2006-02-02       Impact factor: 3.886

7.  A New Chloroplast Protein Is Induced by Growth on Low CO(2) in Chlamydomonas reinhardtii.

Authors:  C B Mason; L J Manuel; J V Moroney
Journal:  Plant Physiol       Date:  1990-06       Impact factor: 8.340

8.  Simplified Procedure for the Isolation of Intact Chloroplasts from Chlamydomonas reinhardtii.

Authors:  C B Mason; S Matthews; T M Bricker; J V Moroney
Journal:  Plant Physiol       Date:  1991-12       Impact factor: 8.340

9.  Transit peptide mutations that impair in vitro and in vivo chloroplast protein import do not affect accumulation of the gamma-subunit of chloroplast ATPase.

Authors:  K L Kindle; S D Lawrence
Journal:  Plant Physiol       Date:  1998-03       Impact factor: 8.340

10.  A type II NAD(P)H dehydrogenase mediates light-independent plastoquinone reduction in the chloroplast of Chlamydomonas.

Authors:  Frédéric Jans; Emmanuel Mignolet; Pierre-Alain Houyoux; Pierre Cardol; Bart Ghysels; Stéphan Cuiné; Laurent Cournac; Gilles Peltier; Claire Remacle; Fabrice Franck
Journal:  Proc Natl Acad Sci U S A       Date:  2008-12-12       Impact factor: 11.205

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