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Literature DB >> 16666230

Na-NMR Studies of the Intracellular Sodium Ion Concentration in the Halotolerant Alga Dunaliella salina.

Abstract

The Intracellular Na(+) concentration in the halotolerant alga Dunaliella salina was measured in intact cells by (23)Na-NMR spectroscopy, utilizing the dysprosium tripolyphosphate complex as a sodium shift reagent, and was found to be 88 +/- 28 millimolar. Intracellular sodium ion content and intracellular volume were the same, within the experimental error, in cells adapted to grow in media containing between 0.1 and 4.0 molar NaCl. These values assume extracellular and intracellular NMR visibilities of the (23)Na nuclei of 100 and 40%, respectively. The relaxation rate of intracellular sodium was enhanced with increasing salinity of the growth medium, in parallel to the intracellular osmosity due to the presence of glycerol, indicating that Na(+) ions and glycerol are codistribbuted within the cell volume.

Entities: Chemical

Year: 1988 PMID： 16666230 PMCID： PMC1054851 DOI： 10.1104/pp.87.4.813

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

8 in total

Review 1. Ion binding in biological systems as studied by NMR spectroscopy.

Authors: S Forsén; B Lindman
Journal: Methods Biochem Anal Date: 1981

2. Coupling between the sodium and proton gradients in respiring Escherichia coli cells measured by 23Na and 31P nuclear magnetic resonance.

Authors: A M Castle; R M Macnab; R G Shulman
Journal: J Biol Chem Date: 1986-06-15 Impact factor: 5.157

3. Energy Facilitated Na Uptake in Excised Corn Roots via P and Na NMR.

Authors: W V Gerasimowicz; S I Tu; P E Pfeffer
Journal: Plant Physiol Date: 1986-07 Impact factor: 8.340

4. Determination of intracellular osmotic volume and sodium concentration in dunaliella.

Authors: A Katz; M Avron
Journal: Plant Physiol Date: 1985-08 Impact factor: 8.340

5. P and C-NMR Studies of the Phosphorus and Carbon Metabolites in the Halotolerant Alga, Dunaliella salina.

Authors: M Bental; M Oren-Shamir; M Avron; H Degani
Journal: Plant Physiol Date: 1988-06 Impact factor: 8.340

6. Measurement of intracellular sodium concentration and sodium transport in Escherichia coli by 23Na nuclear magnetic resonance.

Authors: A M Castle; R M Macnab; R G Shulman
Journal: J Biol Chem Date: 1986-03-05 Impact factor: 5.157

7. Observations of 23Na in frog skin by NMR.

Authors: M M Civan; H Degani; Y Margalit; M Shporer
Journal: Am J Physiol Date: 1983-09

8. 39K, 23Na, and 31P NMR studies of ion transport in Saccharomyces cerevisiae.

Authors: T Ogino; J A den Hollander; R G Shulman
Journal: Proc Natl Acad Sci U S A Date: 1983-09 Impact factor: 11.205

8 in total

1. Na(+)-dependent Ca(2+) transport modulates the secretory response to the Fcepsilon receptor stimulus of mast cells.

Authors: E Rumpel; U Pilatus; A Mayer; I Pecht
Journal: Biophys J Date: 2000-12 Impact factor: 4.033

2. In Vivo pH Regulation by a Na/H Antiporter in the Halotolerant Alga Dunaliella salina.

Authors: A Katz; M Bental; H Degani; M Avron
Journal: Plant Physiol Date: 1991-05 Impact factor: 8.340

3. In vivo 23Na and 31P NMR measurement of a tonoplast Na+/H+ exchange process and its characteristics in two barley cultivars.

Authors: T W Fan; R M Higashi; J Norlyn; E Epstein
Journal: Proc Natl Acad Sci U S A Date: 1989-12 Impact factor: 11.205

7. Cloning and Characterization of Two Putative P-Type ATPases from the Marine Microalga Dunaliella maritima Similar to Plant H⁺-ATPases and Their Gene Expression Analysis under Conditions of Hyperosmotic Salt Shock.

Authors: Dmitrii A Matalin; Dmitrii E Khramov; Alexey V Shuvalov; Vadim S Volkov; Yurii V Balnokin; Larissa G Popova
Journal: Plants (Basel) Date: 2021-12-03

Review 8. Salinity tolerance in plants. Quantitative approach to ion transport starting from halophytes and stepping to genetic and protein engineering for manipulating ion fluxes.

Authors: Vadim Volkov
Journal: Front Plant Sci Date: 2015-10-27 Impact factor: 5.753

8 in total

Na-NMR Studies of the Intracellular Sodium Ion Concentration in the Halotolerant Alga Dunaliella salina.

Review 1. Ion binding in biological systems as studied by NMR spectroscopy.

2. Coupling between the sodium and proton gradients in respiring Escherichia coli cells measured by 23Na and 31P nuclear magnetic resonance.

3. Energy Facilitated Na Uptake in Excised Corn Roots via P and Na NMR.

4. Determination of intracellular osmotic volume and sodium concentration in dunaliella.

5. P and C-NMR Studies of the Phosphorus and Carbon Metabolites in the Halotolerant Alga, Dunaliella salina.

6. Measurement of intracellular sodium concentration and sodium transport in Escherichia coli by 23Na nuclear magnetic resonance.

7. Observations of 23Na in frog skin by NMR.

8. 39K, 23Na, and 31P NMR studies of ion transport in Saccharomyces cerevisiae.

1. Na(+)-dependent Ca(2+) transport modulates the secretory response to the Fcepsilon receptor stimulus of mast cells.

2. In Vivo pH Regulation by a Na/H Antiporter in the Halotolerant Alga Dunaliella salina.

3. In vivo 23Na and 31P NMR measurement of a tonoplast Na+/H+ exchange process and its characteristics in two barley cultivars.

4. Modulation of Na/H Antiporter Activity by Extreme pH and Salt in the Halotolerant Alga Dunaliella salina.

5. Primary structure of the plasma membrane H(+)-ATPase from the halotolerant alga Dunaliella bioculata.

6. Identification of osmoadaptive strategies in the halophile, heterotrophic ciliate Schmidingerothrix salinarum.

7. Cloning and Characterization of Two Putative P-Type ATPases from the Marine Microalga Dunaliella maritima Similar to Plant H⁺-ATPases and Their Gene Expression Analysis under Conditions of Hyperosmotic Salt Shock.

Review 8. Salinity tolerance in plants. Quantitative approach to ion transport starting from halophytes and stepping to genetic and protein engineering for manipulating ion fluxes.