Literature DB >> 16666127

Pathogenesis-related proteins of tomato : p-69 as an alkaline endoproteinase.

P Vera1, V Conejero.   

Abstract

An endoproteinase induced by citrus exocortis viroid has been purified from tomato (Lycopersicon esculentum Mill, cv "Rutgers") leaves. The proteinase corresponds to one of the major pathogenesis-related proteins of tomato plants and was designated proteinase P-69 as it has a molecular weight of 69,000 to 70,000. The proteinase was purified in four steps: (NH(4))(2)SO(4) fractionation, chromatography on Bio-Gel P-60, DEAE-Sepharose chromatography, and casein-Sepharose affinity chromatography. The proteinase had a pH optimum of 8.5 to 9.0 when assayed with either fluorescein thiocarbamoyl derivative (FTC)-casein or FTC-ribulose 1,5-bisphosphate carboxylase/oxygenase as substrates. The proteinase activity was inhibited by pCMB and strongly activated by calcium and magnesium ions as well as by DTT. When analyzed by electrofocusing, the activity showed a pI around 9.0.

Entities:  

Year:  1988        PMID： 16666127      PMCID： PMC1054699          DOI： 10.1104/pp.87.1.58

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  16 in total

1.  A statistical analysis of the relationship between degradative rates and molecular weights of proteins.

Authors:  J F Dice; A L Goldberg
Journal:  Arch Biochem Biophys       Date:  1975-09       Impact factor: 4.013

2.  Physical properties of a minimal infectious RNA(viroik) associated with the exocortis disease.

Authors:  J S Semancik; T J Morris; L G Weathers; B F Rodorf; D R Kearns
Journal:  Virology       Date:  1975-01       Impact factor: 3.616

3.  Synthesis of pathogenesis-related proteins in tobacco is regulated at the level of mRNA accumulation and occurs on membrane-bound polysomes.

Authors:  J P Carr; D C Dixon; D F Klessig
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205

4.  Induction of pathogenesis-related proteins in tobacco leaves.

Authors:  M Matsuoka; Y Ohashi
Journal:  Plant Physiol       Date:  1986-02       Impact factor: 8.340

5.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

6.  A releationship between protein-degradation rates in vivo, isoelectric points, and molecular weights obtained by using density labelling.

Authors:  G J Acton; S Gupta
Journal:  Biochem J       Date:  1979-11-15       Impact factor: 3.857

7.  Cell Walls of Phaseolus vulgaris Leaves Contain the Azocoll-Digesting Proteinase.

Authors:  W van der Wilden; J H Segers; M J Chrispeels
Journal:  Plant Physiol       Date:  1983-11       Impact factor: 8.340

8.  Hydrolytic enzymes in the central vacuole of plant cells.

Authors:  T Boller; H Kende
Journal:  Plant Physiol       Date:  1979-06       Impact factor: 8.340

9.  Source of endoproteolytic activity associated with purified ribulose bisphosphate carboxylase.

Authors:  J L Rosichan; R C Huffaker
Journal:  Plant Physiol       Date:  1984-05       Impact factor: 8.340

10.  A tobacco mosaic virus-induced tobacco protein is homologous to the sweet-tasting protein thaumatin.

Authors:  B J Cornelissen; R A Hooft van Huijsduijnen; J F Bol
Journal:  Nature       Date:  1986 May 29-Jun 4       Impact factor: 49.962
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  26 in total

1.  Primary structure and expression of a pathogen-induced protease (PR-P69) in tomato plants: Similarity of functional domains to subtilisin-like endoproteases.

Authors:  P Tornero; V Conejero; P Vera
Journal:  Proc Natl Acad Sci U S A       Date:  1996-06-25       Impact factor: 11.205

Review 2.  A cut above the rest: the regulatory function of plant proteases.

Authors:  Andreas Schaller
Journal:  Planta       Date:  2004-10-29       Impact factor: 4.116

Review 3.  Pathogenesis related-10 proteins are small, structurally similar but with diverse role in stress signaling.

Authors:  Parinita Agarwal; Pradeep K Agarwal
Journal:  Mol Biol Rep       Date:  2013-12-17       Impact factor: 2.316

4.  Degradation of tobacco pathogenesis-related proteins : evidence for conserved mechanisms of degradation of pathogenesis-related proteins in plants.

Authors:  I Rodrigo; P Vera; L C Van Loon; V Conejero
Journal:  Plant Physiol       Date:  1991-02       Impact factor: 8.340

5.  Characterization of the subtilase gene family in tomato (Lycopersicon esculentum Mill.).

Authors:  J Meichtry; N Amrhein; A Schaller
Journal:  Plant Mol Biol       Date:  1999-03       Impact factor: 4.076

6.  The AVR9 race-specific elicitor of Cladosporium fulvum is processed by endogenous and plant proteases.

Authors:  G F Van den Ackerveken; P Vossen; P J De Wit
Journal:  Plant Physiol       Date:  1993-09       Impact factor: 8.340

7.  cDNA cloning of viroid-induced tomato pathogenesis-related protein P23. Characterization as a vacuolar antifungal factor.

Authors:  I Rodrigo; P Vera; P Tornero; J Hernández-Yago; V Conejero
Journal:  Plant Physiol       Date:  1993-07       Impact factor: 8.340

8.  Age-induced protein modifications and increased proteolysis in potato seed-tubers

Authors: 
Journal:  Plant Physiol       Date:  1999-01       Impact factor: 8.340

9.  Purification, crystallization and preliminary X-ray diffraction analysis of a plant subtilase.

Authors:  Rolf Rose; Franziska Huttenlocher; Anna Cedzich; Markus Kaiser; Andreas Schaller; Christian Ottmann
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2009-04-28

10.  Identification of the viroid-induced tomato pathogenesis-related (PR) protein P23 as the thaumatin-like tomato protein NP24 associated with osmotic stress.

Authors:  I Rodrigo; P Vera; R Frank; V Conejero
Journal:  Plant Mol Biol       Date:  1991-05       Impact factor: 4.076
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