Literature DB >> 16665481

An avidin-biotin solid phase ELISA for femtomole isopentenyladenine and isopentenyladenosine measurements in HPLC purified plant extracts.

B Sotta1, G Pilate, F Pelese, I Sabbagh, M Bonnet, R Maldiney.   

Abstract

A solid phase enzyme immunoassay was developed for isopentenyladenine (iP) and isopentenyladenosine (iPA) quantitation in HPLC purified plant extracts. It was performed on antigen-coated microtitration plates on which bound antibodies were indirectly labeled by the means of a biotinylated goat anti-rabbit antibody and an avidin-alkaline phosphatase conjugate. Less than 3 femtomoles of iP or iPA were easily detected and the measuring range extended from 3 femtomole to 1 picomole. The reproducibility has been tested and intra- and interassay variations did not exceed 5.0%. The specificity of iPA antibodies was good, as determined by cross-reactivity measurements with other adenylic compounds. The specificity of the measurements for iP and iPA was demonstrated by analysis of the immunoreactivity of fractions obtained by HPLC separation of a methanolic tobacco leaf extract.

Entities:  

Year:  1987        PMID： 16665481      PMCID： PMC1056629          DOI： 10.1104/pp.84.3.571

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  20 in total

1.  Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration.

Authors:  S Avrameas; T Ternynck
Journal:  Immunochemistry       Date:  1971-12

2.  Anti-inosine antibodies.

Authors:  H Inouye; S Fuchs; M Sela; U Z Littauer
Journal:  Biochim Biophys Acta       Date:  1971-07-29

3.  Immunoassay of plant constituents. Phytochemical society Tate and Lyle lecture.

Authors:  E W Weiler
Journal:  Biochem Soc Trans       Date:  1983-10       Impact factor: 5.407

4.  The use of avidin-biotin interaction in immunoenzymatic techniques.

Authors:  J L Guesdon; T Ternynck; S Avrameas
Journal:  J Histochem Cytochem       Date:  1979-08       Impact factor: 2.479

5.  Potent and specific inhibition of glutathione synthesis by buthionine sulfoximine (S-n-butyl homocysteine sulfoximine).

Authors:  O W Griffith; A Meister
Journal:  J Biol Chem       Date:  1979-08-25       Impact factor: 5.157

6.  Properties of tobacco (Nicotiana tabacum) cadmium-binding peptide(s). Unique non-metallothionein cadmium ligands.

Authors:  R N Reese; G J Wagner
Journal:  Biochem J       Date:  1987-02-01       Impact factor: 3.857

7.  The effect of glutathione on development in wild carrot suspension cultures.

Authors:  B A Earnshaw; M A Johnson
Journal:  Biochem Biophys Res Commun       Date:  1985-12-31       Impact factor: 3.575

8.  Cadmium in foods and the diet.

Authors:  J C Sherlock
Journal:  Experientia       Date:  1984-02-15

Review 9.  Human health effects of exposure to cadmium.

Authors:  W H Hallenbeck
Journal:  Experientia       Date:  1984-02-15

10.  Accumulation of non-protein metal-binding polypeptides (gamma-glutamyl-cysteinyl)n-glycine in selected cadmium-resistant tomato cells.

Authors:  J C Steffens; D F Hunt; B G Williams
Journal:  J Biol Chem       Date:  1986-10-25       Impact factor: 5.157
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  8 in total

1.  The differential effect of N(6)-benzyl-adenine and N (6)-(Δ (2)-isopentenyl)-adenine on in vitro propagation of Paeonia suffruticosa Andr. is correlated with different hormone contents.

Authors:  L Bouza; M Jacques; B Sotta; E Miginiac
Journal:  Plant Cell Rep       Date:  1993-08       Impact factor: 4.570

2.  Physiological and biochemical responses and microscopic structure changes of Populus tomentosa Carr seedlings to 4-BDE exposure.

Authors:  Man Cai; Yuling Li; Yanling Li; Kejiu Du
Journal:  Environ Sci Pollut Res Int       Date:  2015-05-15       Impact factor: 4.223

3.  Leaf-miners co-opt microorganisms to enhance their nutritional environment.

Authors:  Mélanie Body; Wilfried Kaiser; Géraldine Dubreuil; Jérôme Casas; David Giron
Journal:  J Chem Ecol       Date:  2013-06-27       Impact factor: 2.626

4.  Hormones and Pod Development in Oilseed Rape (Brassica napus).

Authors:  P de Bouille; B Sotta; E Miginiac; A Merrien
Journal:  Plant Physiol       Date:  1989-07       Impact factor: 8.340

5.  Hormone Levels and Apical Dominance in the Aquatic Fern Marsilea drummondii A. Br.

Authors:  G Pilate; L Sossountzov; E Miginiac
Journal:  Plant Physiol       Date:  1989-07       Impact factor: 8.340

6.  Immunodetection and Identification of N-(o-Hydroxybenzylamino)Purine as a Naturally Occurring Cytokinin in Populus x canadensis Moench cv Robusta Leaves.

Authors:  M Strnad; W Peters; E Beck; M Kamínek
Journal:  Plant Physiol       Date:  1992-05       Impact factor: 8.340

7.  Hormonal characterization of a nonrooting naphthalene-acetic Acid tolerant tobacco mutant by an immunoenzymic method.

Authors:  F Pelese; B Megnegneau; B Sotta; L Sossountzov; M Caboche; E Miginiac
Journal:  Plant Physiol       Date:  1989-01       Impact factor: 8.340

8.  Immunocytochemical localization of cytokinins in Craigella tomato and a sideshootless mutant.

Authors:  L Sossountzov; R Maddiney; B Sotta; I Sabbagh; Y Habricot; M Bonnet; E Miginiac
Journal:  Planta       Date:  1988-09       Impact factor: 4.116
  8 in total

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