Literature DB >> 16665225

High speed HPLC analysis of polyamines in plant tissues.

H J Walter1, J M Geuns.   

Abstract

A high speed high performance liquid chromatography (HPLC) method for the quantification of putrescine, spermidine, and spermine in biological samples is described. The dansylation is followed by a sample cleanup. The isocratic HPLC-analysis with acetonitrile:H(2)O (72:28 volume/volume) on 10 centimeter long, 3 micrometer octadecyl silica columns (3 millimeter inner diameter) takes only 4.5 minutes. By our method about 100 analyses can be done in 1 day.

Entities:  

Year:  1987        PMID： 16665225      PMCID： PMC1056336          DOI： 10.1104/pp.83.2.232

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  4 in total

1.  [Microdetermination of spermine and spermidine as 1-dimethylaminonaphthalene-5-sulfonic acid derivtives].

Authors:  N Seiler; M Wiechmann
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1967-10

2.  Analysis of polyamines in higher plants by high performance liquid chromatography.

Authors:  H E Flores; A W Galston
Journal:  Plant Physiol       Date:  1982-03       Impact factor: 8.340

Review 3.  Polyamines.

Authors:  C W Tabor; H Tabor
Journal:  Annu Rev Biochem       Date:  1984       Impact factor: 23.643

4.  Determination of di-and polyamines by high-performance liquid chromatographic separation of their 5-dimethylaminonaphthalene-1-sulfonyl derivatives.

Authors:  N Seiler; B Knödgen
Journal:  J Chromatogr       Date:  1978-01-01
  4 in total
  5 in total

1.  High performance liquid chromatography of the dansyl derivatives of putrescine, spermidine, and spermine.

Authors:  M I Escribano; M E Legaz
Journal:  Plant Physiol       Date:  1988-06       Impact factor: 8.340

2.  Improved method for HPLC analysis of polyamines, agmatine and aromatic monoamines in plant tissue.

Authors:  R D Slocum; H E Flores; A W Galston; L H Weinstein
Journal:  Plant Physiol       Date:  1989       Impact factor: 8.340

3.  Chemical suppressors of mlo-mediated powdery mildew resistance.

Authors:  Hongpo Wu; Mark Kwaaitaal; Roxana Strugala; Ulrich Schaffrath; Paweł Bednarek; Ralph Panstruga
Journal:  Biosci Rep       Date:  2017-12-12       Impact factor: 3.840

4.  Manipulation of arginase expression modulates abiotic stress tolerance in Arabidopsis: effect on arginine metabolism and ROS accumulation.

Authors:  Haitao Shi; Tiantian Ye; Fangfang Chen; Zhangmin Cheng; Yanping Wang; Pingfang Yang; Yansheng Zhang; Zhulong Chan
Journal:  J Exp Bot       Date:  2013-02-01       Impact factor: 6.992

5.  Expression of the Rice Arginase Gene OsARG in Cotton Influences the Morphology and Nitrogen Transition of Seedlings.

Authors:  Zhigang Meng; Zhaohong Meng; Rui Zhang; Chengzhen Liang; Jianmin Wan; Yanling Wang; Honghong Zhai; Sandui Guo
Journal:  PLoS One       Date:  2015-11-03       Impact factor: 3.240
  5 in total

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