Literature DB >> 16665181

Detection of glycosylated and deglycosylated extensin precursors by indirect competitive ELISA.

T A Conrad1, D T Lamport, R Hammerschmidt.   

Abstract

A competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for the rapid quantitation of the glycosylated and deglycosylated forms of the monomeric soluble extensin precursor subunits P1 and P2. A log-linear response range for each kind of precursor in the competition curve was between 0.01 and 100 nanograms per milliliter.

Year:  1987        PMID: 16665181      PMCID: PMC1056287          DOI: 10.1104/pp.83.1.1

Source DB:  PubMed          Journal:  Plant Physiol        ISSN: 0032-0889            Impact factor:   8.340


  5 in total

1.  A method for producing specific antisera with small doses of immunogen.

Authors:  J Vaitukaitis; J B Robbins; E Nieschlag; G T Ross
Journal:  J Clin Endocrinol Metab       Date:  1971-12       Impact factor: 5.958

2.  Hydroxyproline-rich bacterial agglutinin from potato : extraction, purification, and characterization.

Authors:  J E Leach; M A Cantrell; L Sequeira
Journal:  Plant Physiol       Date:  1982-11       Impact factor: 8.340

3.  Purification and Characterization of a Salt-extractable Hydroxyproline-rich Glycoprotein from Aerated Carrot Discs.

Authors:  D A Stuart; J E Varner
Journal:  Plant Physiol       Date:  1980-11       Impact factor: 8.340

4.  Hydroxyproline arabinosides in the plant kingdom.

Authors:  D T Lamport; D H Miller
Journal:  Plant Physiol       Date:  1971-10       Impact factor: 8.340

5.  Determination of the optimal ammonium sulfate concentration for the fractionation of rabbit, sheep, horse, and goat antisera.

Authors:  G A Hebert; P L Pelham; B Pittman
Journal:  Appl Microbiol       Date:  1973-01
  5 in total
  2 in total

1.  Characterization of a xylose-specific antiserum that reacts with the complex asparagine-linked glycans of extracellular and vacuolar glycoproteins.

Authors:  M Laurière; C Laurière; M J Chrispeels; K D Johnson; A Sturm
Journal:  Plant Physiol       Date:  1989-07       Impact factor: 8.340

2.  Antibody localization of extensin in cell walls of carrot storage roots.

Authors:  J P Stafstrom; L A Staehelin
Journal:  Planta       Date:  1988-06       Impact factor: 4.116

  2 in total

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