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Literature DB >> 16664139

Characterization of glutamine synthetase isoforms from chlorella.

Abstract

Ion-exchange chromatography of extracts derived from Chlorella sorokiniana mutant strain (oxygen resistant) yielded two separate activity peaks of glutamine synthetase (GS). GS(I) and GS(II) were purified 220- and 187-fold and have molecular weights of approximately 398,000 and 360,000, respectively. Both enzymes are composed of eight identical subunits with a subunit molecular weight of 47,000 for GS(I) and 43,000 for GS(II). The amino acid composition, catalytic, and immunological properties for both enzymes are similar.

Entities: Chemical Disease Species

Year: 1985 PMID： 16664139 PMCID： PMC1064607 DOI： 10.1104/pp.77.4.791

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

11 in total

1. Post-translational transport into intact chloroplasts of a precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase.

Authors: N H Chua; G W Schmidt
Journal: Proc Natl Acad Sci U S A Date: 1978-12 Impact factor: 11.205

2. The spectrophotometric determination of tyrosine and tryptophan in proteins.

Authors: T W Goodwin; R A Morton
Journal: Biochem J Date: 1946 Impact factor: 3.857

3. A Thioredoxin-Mediated Activation of Glutamine Synthetase and Glutamate Synthase in Synchronous Chlorella sorokiniana.

Authors: R Tischner; A Schmidt
Journal: Plant Physiol Date: 1982-07 Impact factor: 8.340

4. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors: M M Bradford
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

5. Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis.

Authors: J L Hedrick; A J Smith
Journal: Arch Biochem Biophys Date: 1968-07 Impact factor: 4.013

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

7. Glycolate metabolism is under nitrogen control in chlorella.

Authors: R F Beudeker; F R Tabita
Journal: Plant Physiol Date: 1984-07 Impact factor: 8.340

8. Glutamine Synthetases of Higher Plants : Evidence for a Specific Isoform Content Related to Their Possible Physiological Role and Their Compartmentation within the Leaf.

Authors: S F McNally; B Hirel; P Gadal; A F Mann; G R Stewart
Journal: Plant Physiol Date: 1983-05 Impact factor: 8.340

9. Control of photorespiratory glycolate metabolism in an oxygen-resistant mutant of Chlorella sorokiniana.

Authors: R F Beudeker; F R Tabita
Journal: J Bacteriol Date: 1983-08 Impact factor: 3.490

10. Subcellular location of chicken brain glutamine synthetase and comparison with chicken liver mitochondrial glutamine synthetase.

Authors: D D Smith; J W Campbell
Journal: J Biol Chem Date: 1983-10-25 Impact factor: 5.157

6 in total

1. Isolation and characterization of glutamine synthetase genes in Chlamydomonas reinhardtii.

Authors: Q Chen; C D Silflow
Journal: Plant Physiol Date: 1996-11 Impact factor: 8.340

2. Purification and properties of glutamine synthetase from spinach leaves.

Authors: M C Ericson
Journal: Plant Physiol Date: 1985-12 Impact factor: 8.340

3. Purification and Partial Kinetic and Physical Characterization of Two Chloroplast-Localized NADP-Specific Glutamate Dehydrogenase Isoenzymes and Their Preferential Accumulation in Chlorella sorokiniana Cells Cultured at Low or High Ammonium Levels.

Authors: N F Bascomb; R R Schmidt
Journal: Plant Physiol Date: 1987-01 Impact factor: 8.340