Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A Novel Method for the Activation and Solubilization of Coupling Factor 1 from Chloroplasts of Euglena gracilis.

Literature DB >> 16663373

A Novel Method for the Activation and Solubilization of Coupling Factor 1 from Chloroplasts of Euglena gracilis.

Abstract

A method was found for the in situ activation of the latent Ca(2+)-ATPase of the coupling factor from chloroplasts of Euglena gracilis and its resultant solubilization. The activation causes the concomitant solubilization of the enzyme and facilitates easy and nearly complete extraction. The activation consists of lowering the pH of isolated chloroplasts for 2 minutes to pH 4.5 to 4.7 with acetic acid, followed by neutralization. Increases in activity as high as 18-fold can be obtained. The method does not appear to work with chloroplasts from other sources.

Entities: Chemical Species

Year: 1984 PMID： 16663373 PMCID： PMC1066644 DOI： 10.1104/pp.74.1.167

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

11 in total

1. Glyceraldehyde-3-phosphate dehydrogenases from Euglena gracilis. Purification and physical and chemical characterization.

Authors: F E Grissom; J S Kahn
Journal: Arch Biochem Biophys Date: 1975-12 Impact factor: 4.013

2. A NATURALLY OCCURRING INHIBITOR OF MITOCHONDRIAL ADENOSINE TRIPHOSPHATASE.

Authors: M E PULLMAN; G C MONROY
Journal: J Biol Chem Date: 1963-11 Impact factor: 5.157

3. PARTIAL RESOLUTION OF THE ENZYMES CATALYZINE PHOTOPHOSPHORYLATION. I. STIMULATION OF PHOTOPHOSPHORYLATION BY A PREPARATION OF A LATENT, CA++- DEPENDENT ADENOSINE TRIPHOSPHATASE FROM CHLOROPLASTS.

Authors: V K VAMBUTAS; E RACKER
Journal: J Biol Chem Date: 1965-06 Impact factor: 5.157

9. The epsilon Subunit of the Chloroplast Coupling Factor 1 from Euglena gracilis: A Possible Role in Controlling ATPase Activity.

Authors: J S Kahn
Journal: Plant Physiol Date: 1982-08 Impact factor: 8.340

10. Light Activation of Mg-dependent Adenosine 5'-Triphosphatase in Isolated Euglena Chloroplasts.

Authors: I C Chang; J S Kahn
Journal: Plant Physiol Date: 1972-03 Impact factor: 8.340

1 in total

1. Activation of Coupling Factor 1 from Euglena gracilis Chloroplasts : Conditions for Optimal Activation and Their Possible Physiological Significance.

Authors: J S Kahn
Journal: Plant Physiol Date: 1984-06 Impact factor: 8.340

1 in total

A Novel Method for the Activation and Solubilization of Coupling Factor 1 from Chloroplasts of Euglena gracilis.

1. Glyceraldehyde-3-phosphate dehydrogenases from Euglena gracilis. Purification and physical and chemical characterization.

2. A NATURALLY OCCURRING INHIBITOR OF MITOCHONDRIAL ADENOSINE TRIPHOSPHATASE.

3. PARTIAL RESOLUTION OF THE ENZYMES CATALYZINE PHOTOPHOSPHORYLATION. I. STIMULATION OF PHOTOPHOSPHORYLATION BY A PREPARATION OF A LATENT, CA++- DEPENDENT ADENOSINE TRIPHOSPHATASE FROM CHLOROPLASTS.

4. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.

5. Protein measurement with the Folin phenol reagent.

6. Isolation of a possible coupling factor for photophosphorylation from chloroplasts of Euglena gracilis.

7. Isolation and properties of a chloroplast coupling factor and heat-activated adenosine triphosphatase.

8. Requirement of the delta subunit of chloroplast coupling factor 1 for photophosphorylation.

9. The epsilon Subunit of the Chloroplast Coupling Factor 1 from Euglena gracilis: A Possible Role in Controlling ATPase Activity.

10. Light Activation of Mg-dependent Adenosine 5'-Triphosphatase in Isolated Euglena Chloroplasts.

1. Activation of Coupling Factor 1 from Euglena gracilis Chloroplasts : Conditions for Optimal Activation and Their Possible Physiological Significance.