Gas Exchange and Phytoluminography of Single Red Kidney Bean Leaves during Periods of Induced Stomatal Oscillations: A Demonstration of an Integrated, Spatially Resolving Physiometric Technique.

This report examines the capabilities of a new approach to the study of gas exchange and electron transport properties of single, intact leaves. The method combines conventional aspects of analysis with an image intensification system that records the spatial distribution of delayed light emission (DLE) over single leaf surfaces. The combined system was used to investigate physiological perturbations induced by exposure of single leaves of Phaseolus vulgaris cv ;California Light Red' to a combination of SO(2) (0.5 microliters per liter) and ozone (0.1 microliters per liter). Exposure of one-half of a leaf to this combination induced DLE and stomatal oscillations, but only in the half of the leaf exposed to the combined gases. Examination of phytoluminographs taken during these oscillations revealed distinct leaf patches where the greatest changes in DLE intensity occurred. This phenomenon is interpreted to be evidence that control of stomatal activity of intact plant leaves occurs within discrete leaf areas defined within the vascular network.