Intracellular distribution of free sugars in quiescent cottonseed.

Quiescent cottonseeds (Gossypium hirsutum L.) were fractionated non-aqueously to protein bodies and nonparticulate cytoplasm for analyses of sugar contents. Nonparticulate cytoplasm was more than 3-fold richer in total free sugars than were protein bodies, but based on the content of cytoplasm in each fraction, sugars were distributed almost equally in them. Thin-layer chromatography and high-pressure liquid chromatography revealed five sugars: sucrose, raffinose, tetrasaccharides I and II, and stachyose. Raffinose and stachyose were located mainly, and tetrasaccharide I totally, in nonparticulate cytoplasm compared to protein bodies; sucrose and tetrasaccharide II were distributed about equally in each fraction.