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Literature DB >> 16662639

Isolation of spinach leaf peroxisomes in 0.25 molar sucrose solution by percoll density gradient centrifugation.

Abstract

A procedure for isolating spinach (Spinacia oleracea L.) leaf peroxisomes in 0.25 molar sucrose solution by Percoll density gradient centrifugation followed by removal of the Percoll by washing and centrifugation was established. The preparation contains more than 90% peroxisomes as intact organelles with no detectable chlorophyll or cytochrome oxidase contamination. The peroxisomes are stable at 0 to 4 degrees C or 25 degrees C for at least 2 hours.

Entities: Chemical Species

Year: 1982 PMID： 16662639 PMCID： PMC1065851 DOI： 10.1104/pp.70.4.1210

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

6 in total

Review 1. Peroxisomes (microbodies and related particles).

Authors: C De Duve; P Baudhuin
Journal: Physiol Rev Date: 1966-04 Impact factor: 37.312

2. Oxidation of NADH in Glyoxysomes by a Malate-Aspartate Shuttle.

Authors: I J Mettler; H Beevers
Journal: Plant Physiol Date: 1980-10 Impact factor: 8.340

3. Organelle-specific Isozymes of Aspartate-alpha-Ketoglutarate Transaminase in Spinach Leaves.

Authors: A H Huang; K D Liu; R J Youle
Journal: Plant Physiol Date: 1976-07 Impact factor: 8.340

4. Induction of peroxisomal beta-oxidation in rat liver by high-fat diets.

Authors: C E Neat; M S Thomassen; H Osmundsen
Journal: Biochem J Date: 1980-01-15 Impact factor: 3.857

5. Cell-free synthesis of watermelon glyoxysomal malate dehydrogenase: a comparison with the mitochondrial isoenzyme.

Authors: B Hock; C Gietl
Journal: Ann N Y Acad Sci Date: 1982 Impact factor: 5.691

6. Localization of enzymes within microbodies.

Authors: A H Huang; H Beevers
Journal: J Cell Biol Date: 1973-08 Impact factor: 10.539

6 in total

7 in total

6. Compartmentation studies on spinach leaf peroxisomes : evidence for channeling of photorespiratory metabolites in peroxisomes devoid of intact boundary membrane.

Authors: R Heupel; T Markgraf; D G Robinson; H W Heldt
Journal: Plant Physiol Date: 1991-07 Impact factor: 8.340

7. Reactivity of glyoxylate with hydrogen perioxide and simulation of the glycolate pathway of C3 plants and Euglena.

Authors: A Yokota; S Kitaoka; K Miura; A Wadano
Journal: Planta Date: 1985-07 Impact factor: 4.116

7 in total

Isolation of spinach leaf peroxisomes in 0.25 molar sucrose solution by percoll density gradient centrifugation.

Review 1. Peroxisomes (microbodies and related particles).

2. Oxidation of NADH in Glyoxysomes by a Malate-Aspartate Shuttle.

3. Organelle-specific Isozymes of Aspartate-alpha-Ketoglutarate Transaminase in Spinach Leaves.

4. Induction of peroxisomal beta-oxidation in rat liver by high-fat diets.

5. Cell-free synthesis of watermelon glyoxysomal malate dehydrogenase: a comparison with the mitochondrial isoenzyme.

6. Localization of enzymes within microbodies.

1. Isolation of mitochondria from soybean leaves on discontinuous percoll gradients.

2. Effects of CO(2) Enrichment and Carbohydrate Content on the Dark Respiration of Soybeans.

3. Metabolism of glycolate and glyoxylate in intact spinach leaf peroxisomes.

4. Glyoxylate transamination in intact leaf peroxisomes.

5. Purification of peroxisomes and mitochondria from spinach leaf by percoll gradient centrifugation.

6. Compartmentation studies on spinach leaf peroxisomes : evidence for channeling of photorespiratory metabolites in peroxisomes devoid of intact boundary membrane.

7. Reactivity of glyoxylate with hydrogen perioxide and simulation of the glycolate pathway of C3 plants and Euglena.