Literature DB >> 16662332

Lysine-ketoglutarate reductase activity in developing maize endosperm.

P Arruda1, L Sodek, W J da Silva.   

Abstract

Lysine-ketoglutarate reductase activity was detected and characterized in the developing endosperm of maize (Zea mays L.). The enzyme showed specificity for its substrates: lysine, alpha-ketoglutarate, and NADPH. Formation of the reaction product saccharopine was demonstrated. The pH optimum of the enzyme was close to 7, and the K(m) for lysine and alpha-ketoglutarate were 5.2 and 1.8 millimolar, respectively.

Entities:  

Year:  1982        PMID： 16662332      PMCID： PMC426341          DOI： 10.1104/pp.69.4.988

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  7 in total

1.  Saccharopine cleavage by a dehydrogenase of human liver.

Authors:  J Hutzler; J Dancis
Journal:  Biochim Biophys Acta       Date:  1970-05-13

2.  Conversion of lysine to saccharopine by human tissues.

Authors:  J Hutzler; J Dancis
Journal:  Biochim Biophys Acta       Date:  1968-04-16

3.  A mixed layer for separation of amino acids by thin-layer chromatography.

Authors:  N A Turner; R J Redgwell
Journal:  J Chromatogr       Date:  1966-01

4.  Saccharopine, an intermediate of the aminoadipic acid pathway of lysine biosynthesis. IV. Saccharopine dehydrogenase.

Authors:  P P Saunders; H P Broquist
Journal:  J Biol Chem       Date:  1966-07-25       Impact factor: 5.157

5.  Lysine Catabolism in Barley (Hordeum vulgare L.).

Authors:  B L Møller
Journal:  Plant Physiol       Date:  1976-05       Impact factor: 8.340

6.  General and lysin specific control of saccharopine dehydrogenase levels in the yeast Saccharomycopsis lipolytica.

Authors:  C M Gaillardin; L Poirier; A M Ribet; H Heslot
Journal:  Biochimie       Date:  1979       Impact factor: 4.079

7.  Incorporation of leucine-14C and lysine-14C into protein in the developing endosperm of normal and opaque-2 corn.

Authors:  L Sodek; C M Wilson
Journal:  Arch Biochem Biophys       Date:  1970-09       Impact factor: 4.013
  7 in total
  6 in total

1.  Lysine degradation through the saccharopine pathway in mammals: involvement of both bifunctional and monofunctional lysine-degrading enzymes in mouse.

Authors:  F Papes; E L Kemper; G Cord-Neto; F Langone; P Arruda
Journal:  Biochem J       Date:  1999-12-01       Impact factor: 3.857

2.  Purification and characterization of bifunctional lysine-ketoglutarate reductase/saccharopine dehydrogenase from developing soybean seeds.

Authors:  D Miron; S Ben-Yaacov; D Reches; A Schupper; G Galili
Journal:  Plant Physiol       Date:  2000-06       Impact factor: 8.340

3.  Partial purification and characterization of lysine-ketoglutarate reductase in normal and opaque-2 maize endosperms.

Authors:  M R Brochetto-Braga; A Leite; P Arruda
Journal:  Plant Physiol       Date:  1992-03       Impact factor: 8.340

4.  The role of opaque2 in the control of lysine-degrading activities in developing maize endosperm.

Authors:  E L Kemper; G C Neto; F Papes; K C Moraes; A Leite; P Arruda
Journal:  Plant Cell       Date:  1999-10       Impact factor: 11.277

5.  Sodium fluoride causes oxidative stress and apoptosis in the mouse liver.

Authors:  Yujiao Lu; Qin Luo; Hengmin Cui; Huidan Deng; Ping Kuang; Huan Liu; Jing Fang; Zhicai Zuo; Junliang Deng; Yinglun Li; Xun Wang; Ling Zhao
Journal:  Aging (Albany NY)       Date:  2017-06-27       Impact factor: 5.682

6.  Specialist and Generalist Fungal Parasites Induce Distinct Biochemical Changes in the Mandible Muscles of Their Host.

Authors:  Shanshan Zheng; Raquel Loreto; Philip Smith; Andrew Patterson; David Hughes; Liande Wang
Journal:  Int J Mol Sci       Date:  2019-09-17       Impact factor: 5.923
  6 in total

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