Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Reassessment of plasma angiotensins measurement: effects of protease inhibitors and sample handling procedures.

Literature DB >> 1666184

Reassessment of plasma angiotensins measurement: effects of protease inhibitors and sample handling procedures.

K Kohara¹, Y Tabuchi, P Senanayake, K B Brosnihan, C M Ferrario.

Abstract

Characterization of C- and N-terminal forms of angiotensin (Ang) peptides mandated assessment of methods to determine plasma levels. 125I-Ang I, 125I-Ang II, and 125I-Ang(1-7) were added to blood samples in the presence of protease inhibitors. Ethylenediaminetetraacetic acid (EDTA) inhibited the conversion of 125I-Ang I to 125I-Ang II. o-Phenanthroline and EDTA (EDTA + o-Ph) did not eliminate [des-Asp1] fragments or 125I-Ang(1-7). The combination of EDTA + o-Ph and pepstatin A or 4-(chloromercuri) benzoic acid (PCMB) significantly reduced 125I-Ang(1-7) generation. Only PCMB plus EDTA + o-Ph eliminated [des-Asp1] fragments. Authentic plasma values of Ang peptides require the correct choice of protease inhibitors.

Entities: Chemical Gene

Mesh：

Substances：

Year: 1991 PMID： 1666184 DOI： 10.1016/0196-9781(91)90070-6

Source DB: PubMed Journal: Peptides ISSN： 0196-9781 Impact factor: 3.750

Keyword Cloud
Cited

26 in total

Reassessment of plasma angiotensins measurement: effects of protease inhibitors and sample handling procedures.

1. Simultaneous analysis of angiotensin peptides by LC-MS and LC-MS/MS: metabolism by bovine adrenal endothelial cells.

Review 2. Biochemical evaluation of the renin-angiotensin system: the good, bad, and absolute?

3. Altered systemic hemodynamic and baroreflex response to angiotensin II in postural tachycardia syndrome.

4. Longitudinal study of angiotensin peptides in normal and pre-eclamptic pregnancy.

5. Antenatal betamethasone exposure alters renal responses to angiotensin-(1-7) in uninephrectomized adult male sheep.

6. Abnormalities of angiotensin regulation in postural tachycardia syndrome.

7. Hemodynamic and hormonal patterns of untreated essential hypertension in men and women.

8. Hemodynamic, morphometric and autonomic patterns in hypertensive rats - Renin-Angiotensin system modulation.

9. Decreased cardiac Ang-(1-7) is associated with salt-induced cardiac remodeling and dysfunction.

10. Phase I and pharmacokinetic study of angiotensin-(1-7), an endogenous antiangiogenic hormone.