Literature DB >> 1666114

A rapid method of sample preparation for detection of DNA viruses in human serum by polymerase chain reaction.

N Frickhofen1, N S Young.   

Abstract

A rapid method of serum treatment is described that can be used for the detection of viral DNA by polymerase chain reaction (PCR). The key feature of the assay is inactivation of inhibitory serum factors by controlled heating of serum. This method avoids DNA extraction. It is very fast and limited sample handling decreases the chances of contamination by exogenous DNA. It has been successfully used for demonstration of parvovirus B19, hepatitis B virus, and human cytomegalovirus DNA in patient sera. Since pathological components in sera from patients with various diseases do not interfere in the assay, it can be used as a sensitive and safe screening assay for DNA viruses in a routine clinical setting.

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Year:  1991        PMID： 1666114     DOI： 10.1016/0166-0934(91)90086-f

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  8 in total

1.  Comparative evaluation of virological and serological methods in prenatal diagnosis of parvovirus B19 fetal hydrops.

Authors:  M Zerbini; M Musiani; G Gentilomi; S Venturoli; G Gallinella; R Morandi
Journal:  J Clin Microbiol       Date:  1996-03       Impact factor: 5.948

2.  Comparison of hepatitis B virus DNA extractions from serum by the QIAamp blood kit, GeneReleaser, and the phenol-chloroform method.

Authors:  A Kramvis; S Bukofzer; M C Kew
Journal:  J Clin Microbiol       Date:  1996-11       Impact factor: 5.948

3.  PCR detection of cytomegalovirus DNA in serum as a diagnostic test for congenital cytomegalovirus infection.

Authors:  C T Nelson; A S Istas; M K Wilkerson; G J Demmler
Journal:  J Clin Microbiol       Date:  1995-12       Impact factor: 5.948

4.  Detection of human herpesvirus 6 DNA in serum by a microplate PCR-hybridization assay.

Authors:  C Osiowy; I Prud'homme; M Monette; S Zou
Journal:  J Clin Microbiol       Date:  1998-01       Impact factor: 5.948

5.  A case of pure red cell aplasia: follow-up on different immunosuppressive regimens.

Authors:  M Baldus; M Möller; H Walter; H Brass; W Queisser
Journal:  Clin Investig       Date:  1994-12

6.  An improved method for the isolation of hepatitis B virus DNA from human serum.

Authors:  Harish Changotra; Prabodh K Sehajpal
Journal:  Indian J Virol       Date:  2013-08-08

7.  Rapid diagnosis of enterovirus infection by magnetic bead extraction and polymerase chain reaction detection of enterovirus RNA in clinical specimens.

Authors:  P Muir; F Nicholson; M Jhetam; S Neogi; J E Banatvala
Journal:  J Clin Microbiol       Date:  1993-01       Impact factor: 5.948

8.  The relationship between capsid protein (VP2) sequence and pathogenicity of Aleutian mink disease parvovirus (ADV): a possible role for raccoons in the transmission of ADV infections.

Authors:  K L Oie; G Durrant; J B Wolfinbarger; D Martin; F Costello; S Perryman; D Hogan; W J Hadlow; M E Bloom
Journal:  J Virol       Date:  1996-02       Impact factor: 5.103
  8 in total

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