| Literature DB >> 16661137 |
R H Hageman1, A J Reed, R A Femmer, J H Sherrard, M J Dalling.
Abstract
Experiments were carried out to clarify problems encountered in measuring metabolic and storage pool sizes of nitrate in wheat leaf sections with an in vivo nitrate reductase assay. The leaf sections were from seedlings grown on 15 millimolar nitrate. Data obtained show that the cessation of nitrite accumulation, used as an index of the active nitrate pool size, could be caused by lack of anaerobiosis in the assay system, the lack of energy for nitrate reduction, or a loss of nitrate reductase activity. Availability of nitrate was never the limiting factor in this system. It is concluded that pool sizes of nitrate cannot be determined in wheat leaves with the in vivo assays employed.Entities:
Year: 1980 PMID: 16661137 PMCID: PMC440260 DOI: 10.1104/pp.65.1.27
Source DB: PubMed Journal: Plant Physiol ISSN: 0032-0889 Impact factor: 8.340