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Literature DB >> 16660958

Phytochrome radioimmunoassay.

Abstract

A phytochrome radioimmunoassay with a detection limit of about 2 nanograms has been developed. The radioimmunoassay does not suffer from the potential drawbacks of the commonly used spectral assay and requires less than 1 microliter of crude extract from dark-grown plants for quantitation of phytochrome. Measurement of phytochrome in crude extracts by radioimmunoassay gives values about 25% greater than those obtained by spectral assay. The amount of phytochrome detected in crude extracts of light-grown oats by radioimmunoassay is approximately 1% of that detected in comparable extracts from dark-grown oats. General interference by crude plant extracts with radioimmunoassays was also observed and corrected for.

Entities: Species

Year: 1979 PMID： 16660958 PMCID： PMC543080 DOI： 10.1104/pp.64.2.327

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

15 in total

10. Immunochemical detection with rabbit polyclonal and mouse monoclonal antibodies of different pools of phytochrome from etiolated and green Avena shoots.

Authors: Y Shimazaki; L H Pratt
Journal: Planta Date: 1985-06 Impact factor: 4.116

Phytochrome radioimmunoassay.

1. Immunochemical and spectroscopic evidence for protein conformational changes in phytochrome transformations.

2. Immunological Determination of the Relationship between Large and Small Sizes of Phytochrome.

3. Immunocytochemical localization of phytochrome.

4. Use of I-labeled phytochrome to quantitate phytochrome binding to membranes of Avena sativa.

5. DETECTION, ASSAY, AND PRELIMINARY PURIFICATION OF THE PIGMENT CONTROLLING PHOTORESPONSIVE DEVELOPMENT OF PLANTS.

6. Studies on the protein comformation of phytochrome.

7. Radioactive labeling of proteins in vitro.

8. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

9. An immunochemical characterization of the phytochrome destruction reaction.

10. Comparative immunochemistry of phytochrome.

1. Oat Phytochrome Is Biologically Active in Transgenic Tomatoes.

2. Autoregulatory control of translatable phytochrome mRNA levels.

3. Phytochrome Modification and Light-enhanced, In Vivo-induced Phytochrome Pelletability.

4. Phytochrome immunoaffinity purification.

5. Phytochrome Pelletability Induced by Irradiation in Vivo: TEST FOR IN VITRO BINDING OF ADDED [S]PHYTOCHROME.

6. Production and purification of monoclonal antibodies to Pisum and Avena phytochrome.

7. Analysis of phytochrome kinetics in light-grown Avena sativa L. seedlings.

8. The influence of light quality on the phytochrome content of light grown Sinapis alba L. and Phaseolus aureus Roxb.

9. Phytochrome quantitation in crude extracts of Avena by enzyme-linked immunosorbent assay with monoclonal antibodies.

10. Immunochemical detection with rabbit polyclonal and mouse monoclonal antibodies of different pools of phytochrome from etiolated and green Avena shoots.