Literature DB >> 16660642

Thymidine phosphorylation in wheat: analysis of phosphate transfer from ATP to thymidine.

J Hofman1, O J Schwarz.   

Abstract

Extracts of wheat (Triticum vulgare Vill. [Triticum aestivum L.] var. Lemhi) seedlings contain thymidine-phosphorylating activity with ATP, ADP, or AMP and nucleotide hydrolase activity (ATP --> --> AMP). The synthesis of [(32)P]dTMP exclusively from [alpha-(32)P]ATP with none detectable from [gamma-(32)P]ATP demonstrates the absence of thymidine kinase and the presence of nucleoside phosphotransferase as the only observable thymidine-phosphorylating enzyme.

Entities:  

Year:  1978        PMID： 16660642      PMCID： PMC1092258          DOI： 10.1104/pp.62.6.930

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  8 in total

1.  DEOXYTHYMIDINE KINASE OF ESCHERICHIA COLI. I. PURIFICATION AND SOME PROPERTIES OF THE ENZYME.

Authors:  R OKAZAKI; A KORNBERG
Journal:  J Biol Chem       Date:  1964-01       Impact factor: 5.157

2.  Thymidine-phosphorylating Activity in gamma-Plantlets: Separation of Onset of Activity from Deoxyribonucleic Acid Synthesis.

Authors:  O J Schwarz; A H Haber
Journal:  Plant Physiol       Date:  1973-05       Impact factor: 8.340

3.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

4.  A nucleotide phosphotransferase from Escherichia coli. Purification and properties.

Authors:  E F Brunngraber; E Chargaff
Journal:  Biochemistry       Date:  1973-07-31       Impact factor: 3.162

5.  Modes of nucleoside phosphorylation in plants: studies on the apparent thymidine kinase and true uridine kinase of seedlings.

Authors:  Q I Deng; D H Ives
Journal:  Biochim Biophys Acta       Date:  1972-08-25

6.  A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.

Authors:  I M Glynn; J B Chappell
Journal:  Biochem J       Date:  1964-01       Impact factor: 3.857

7.  Ion-exchange thin-layer chromatography. XV. Preparation, properties and applications of paper-like PEI-cellulose sheets.

Authors:  K Randerath; E Randerath
Journal:  J Chromatogr       Date:  1966-04

8.  Chromatographic separation of pyridine and adenine nucleotides on thin layers of poly(ethyleneimine) cellulose.

Authors:  R A Barton; A Schulman; E L Jacobson; M K Jacobson
Journal:  J Chromatogr       Date:  1977-01-11
  8 in total

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